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目的：研究脾切除对肝纤维化大鼠肝脏TGFβ1 的表达和血清TGFβ1 水平的影响，探讨脾切除在肝纤维化中的意义。方法：用CCL4 建立50 例肝纤维化大鼠模型。于建模第3 周,6 周, 及8 周分别取大鼠肝脏和脾脏标本。用免疫组化SP 方法测定其 TGFβ1 的表达，HE 和姬姆萨染色检测肝纤维化。应用双抗体夹心ELISA 方法测定15 例模型大鼠行脾脏切除前后的血清 TGFβ1 水平，以及15 例对照组大鼠的血清TGFβ1 水平，并于术后4 周取两组大鼠的肝脏标本，用免疫组化SP 方法测定其 TGFβ1 的表达。应用CMIAS8 彩色图像系统对阳性目标进行分析和处理。结果：随着肝纤维化程度的进展，大鼠肝脏和脾脏 TGFβ1 的表达也随之增加（P<0.01）。脾切除组大鼠其血清TGF-β1 的水平显著低于对照组大鼠（P<0.05），且脾切除组大鼠肝脏 TGFβ1 的表达低于对照组大鼠（P<0.05）。结论：脾切除术在一定程度上可延缓肝纤维化的发展。

英文摘要:

Objective: To study the effects of splenenctomy on the expression of TGFβ 1 in the liver and the serum level of TGFβ 1 of the rats with hepatic firosis and discuss the effects of splenectomy on hepaic firosis. Methods: By hypodermic injection CCL4,we established 50 rat models with hepatic fibrosis,then took different samples of the livers and spleens of the rats in 3,6,and 8weeks. We also took liver samples of the rats with splenectomy after 4 weeks.The expressions of TGFβ1 in the spleen and liver were detected by immunohistochemistry technique and the degree of hepatic fibrosis were detected by HE and Masson's staining. IOD for TGFβ1 were measured by CMIAS8 computer colour image analysis systems.The serum levels of TGFβ1 were analyzed by ELISA technique. Results: The results indicated that with the development of hepatic fibrosis ,the expression of TGFβ1 in the liver and spleen increased (P<0.01). The serum levels of TGFβ1 of the rats after splenectomy were significantly lower than which of the rats without splenectomy （P<0.05）. And the expressions of TGFβ1 in the liver of the rats with splenectomy were less than which of the rats without splenectomy（P<0.05）. Conclusions: we can infer that to some extent , splenectomy may delay the development of hepatic fibrosis.

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