| 蔡承魁贠喆张涛姜扩高杰裘秀春马保安.miR-15a 和miR-16-1 模拟物对人骨肉瘤细胞系SOSP-9607 凋亡
和增殖的影响[J].,2011,11(8):1417-1419 |
| miR-15a 和miR-16-1 模拟物对人骨肉瘤细胞系SOSP-9607 凋亡
和增殖的影响 |
| Effects of miR-15a and miR-16-1 mimics on Apoptosis and Proliferation ofHuman Osteosarcoma Cell Line SOSP-9607 |
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| DOI： |
| 中文关键词: 骨肉瘤 成骨肉瘤细胞系SOSP-9607 miR-15a miR-16-1 细胞凋亡 细胞增殖 |
| 英文关键词: Osteosarcoma SOSP-9607 cell line miR-15a miR-16-1 cell apoptosis cell proliferation |
| 基金项目:国家自然科学基金项目（81072194） |
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| 中文摘要: |
| 目的：探讨miR-15a 和miR-16-1 模拟物对于人骨肉瘤细胞系SOSP-9607 凋亡和增殖的影响。方法：将SOSP-9607 细胞分为
实验组和对照组。实验组分为miR-15a 组、miR-16-1 组、miR-15a+miR-16-1 组。以miR-15a 组为例，采用miR-15a 模拟物
（hsa-miR-15a mimics）上调SOSP-9607 细胞内的miR-15a 表达量。对照组分为阴性对照组和空白对照组。采用流式细胞仪测定细
胞凋亡率，四甲基偶氮唑蓝（MTT）法测定细胞增殖，并计算细胞增殖效率。结果：通过统计学分析，实验组凋亡率与阴性对照组
凋亡率相比明显增高（P<0.05）；实验组的细胞增殖率明显低于对照组(P<0.05)。结论：上调SOSP-9607 细胞内miR-15a 和
miR-16-1 的表达量可促进SOSP-9607 细胞的凋亡并抑制其增殖。 |
| 英文摘要: |
| Objective : To investigate the effects of miR-15a and miR-16-1 mimics on apoptosis and proliferation of human
osteosarcoma cell line SOSP-9607. Methods: Cultured SOSP-9607 cells were divided into experimental group and control group: the
experimental groups contained miR-15a group, miR-16-1 group and miR-15a+miR-16-1 group. Take miR-15a group for example, we use
hsa-miR-15a mimics to upgrade the expression of miR-15a in SOSP-9607 cells. The control groups contained negative control and the
blank control. The apoptosis were measured by flow cytometry, and MTT assay was used to examine the proliferation efficiency of
human osteosarcoma cell line SOSP-9607. Results: The apoptosis ratio of experimental groups was higher than the control groups
（P<0.05）and the proliferation efficiency of experimental groups was higher than control groups（P<0.05）. Conclusions: the miR-15a and
miR-16-1 plays an important role in cell apoptosis and proliferation in SOSP-9607 cells. |
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