| 安艳新任贵田启飞赵青川.单克隆抗体MGd1 对胃癌SGC-7901 细胞增殖和凋亡的影响[J].,2011,11(8):1409-1412 |
| 单克隆抗体MGd1 对胃癌SGC-7901 细胞增殖和凋亡的影响 |
| Effect of Monoclonal AntibodyMGd1 on Proliferation and Apoptosis ofGastric Cancer Cell SGC-7901 |
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| DOI： |
| 中文关键词: 单克隆抗体 MGd1 增殖 凋亡 |
| 英文关键词: Monoclonal antibody MGd1 Proliferation Apoptosis |
| 基金项目:国家重点基础发展计划项目（973 计划）(No. 2009CB521705)；国家自然科学
基金（No.30971337） |
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| 中文摘要: |
| 目的：研究不同浓度的MGd1 对体外培养胃癌细胞SGC-7901 增殖及凋亡的影响。方法：采用MTT 法测定不同浓度MGd1
对SGC-7901 生长抑制作用；流式细胞术（FCM）进行细胞凋亡分析。激光共聚焦显微镜观察MGd1 抗原（MGd1-Ag）的亚细胞定
位。结果：MTT 结果显示不同浓度的MGd1 均对SGC-7901 细胞产生明显的抑制效应(P=0.02)；流式细胞术分析发现MGd1 可诱
导SGC-7901 发生凋亡并呈浓度和时间依赖性(P<0.01)；共聚焦显微镜结果显示MGd1-Ag 主要定位于细胞膜上。结论：以上结果
证实胃癌特异性单抗MGd1 可抑制SGC-7901 的增殖并促进凋亡发生。它可能通过与细胞膜上抗原特异性结合，影响下游信号传
导，从而发挥抑制效应。 |
| 英文摘要: |
| Objective: To investigate the effects of MGd1 with different concentration on the cell proliferation and apoptosis of
gastric cancer cell line SGC-7901. Methods: Cell proliferation was detected by MTT assay and cell apoptosis was analyzed by flow
cytometry. Subcellular localization of MGb2-Ag in SGC-7901 was detected by immunofluorescence staining using Laser scanning
confocal microscope. Results: MGd1 could inhibit the cell proliferation and promote apoptosis of SGC7901 in dose- and time-dependent
manners. The localization of MGd1-Ag was detected under confocal microscopy, and it showed that MGd1-Ag was mainly located in the
membrane. Conclusion: MGd1 inhibits cell proliferation and promotes apoptosis of SGC7901 by banding with MGd1-Ag. |
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