| 彭文芳1，2 戴爱国2△ 胡瑞成2 朱黎明2.支气管哮喘豚鼠肺组织中KLF2 的表达及意义[J].,2011,11(7):1235-1240 |
| 支气管哮喘豚鼠肺组织中KLF2 的表达及意义 |
| Expression of KLF2 in Lung Tissue of Guinea Pigs with BronchialAsthma and Its Significance |
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| DOI： |
| 中文关键词: 支气管哮喘 锌指Krüppel 样转录因2 |
| 英文关键词: Bronchial Asthma Krüppel-like factor transcription factor 2 |
| 基金项目:湖南省医药卫生科研计划课题项目（B2010-089）；2005 年第八批省级科技计划项目（05JT1024）；
湖南省医药卫生科研计划课题项目（B2007169） |
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| 中文摘要: |
| 目的：探讨锌指Krüppel 样转录因2（KLF2）在豚鼠支气管哮喘肺组织中的表达及意义。方法：30 只健康雄性豚鼠，按随机
数字表法分为对照组(A 组)、哮喘组(B 组)及地塞米松治疗组(C 组)，每组10 只。卵清蛋白致敏法复制哮喘模型。观察豚鼠肺组织
病理学改变，BALF 细胞总数及分类计数；采用原位杂交和RT-PCR 检测肺组织中KLF2 的mRNA 表达情况，免疫组化和Western
blot 检测肺组织中KLF2 的蛋白表达水平。结果：（1）哮喘组豚鼠BALF 中细胞总数、嗜酸性粒细胞百分比（EOS%）、中性粒细胞百
分比（NEU%）显著高于对照组（P<0.01），哮喘组肺组织可见大量炎症细胞浸润，及明显气道重塑改变，而地塞米松治疗组BALF
炎细胞浸润及肺组织病理改变较哮喘组明显减轻；（2）KLF2mRNA 和蛋白在哮喘组表达显著低于对照组，在地塞米松治疗组中的
表达明显高于哮喘组,3 组差异均有统计学意义（P 均<0.01）；（3）KLF2 蛋白以及mRNA 与肺泡灌洗液炎细胞总数及EOS%,
NEU%呈负相关。结论：KLF2 在支气管哮喘急性发作期模型中表达明显下降，而地塞米松治疗后KLF2 的表达上调，提示KLF2
可能在支气管哮喘的发病机制与防治中起重要作用。 |
| 英文摘要: |
| Objective: To investigate the expression and significance of KLF2 in lung tissue of guinea pigs with Bronchial Asthma.
Methods: Thirty healthy male guinea pigs were randomly divided into 4 groups: the control group ( group A) , asthmatic group (group
B)，Dexamethasone group (group C) , 10 guinea pigs in each group. The asthmatic model was established by the ovalbumin challenge
method. Before determining the total number of BALF cells and cells classification，the changes in lung histopathology were observed.
Expressions of KLF2 mRNA in lung tissue were assayed by in situ hybridization and by RT-PCR; Expressions of KLF2 protein were
detected by immunohischemistry and by Western blot. Results: (1)The total number of inflammatory cells and EOS%, NEU% in BALF
of asthma group were significantly higher than those of control group (P<0.01), and large numbers of inflammatory cell infiltration and
obviously bronchiole remodeling response could be seen in this group .However, all these changes were obvious decreased after Dexamethasone
treatment. (2) Protein and mRNA expressions of KLF2 in asthma group significantly higher than those in control group (P<0.
01) , and those in Dexamethasone group was markedly increased compared to asthma group (all P < 0.01). (3) Linear correlation analysis
showed that KLF2mRNA and protein was negatively correlated with EOS%, NEU% and the total number of inflammatory cells in BALF
( r = 0.524, P < 0.01). Conclusions: In acute exacerbation of Bronchial Asthma model induced by ovalbumin, the study showed that asthmatic
guinea pigs have significantly decreased expressions of KLF2,which up-regulated by Dexamethasone treatment group, suggesting
that KLF2 may play an important role in the pathogenesis and prevention of Bronchial Asthma. |
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