| 李晖刘一浓王菲魏蓓蓓周毅杜子秀吴飞.疏水修饰聚阳离子高分子SP-Chol 在COS-7 细胞中转染和毒性的研究[J].,2011,11(7):1205-1207 |
| 疏水修饰聚阳离子高分子SP-Chol 在COS-7 细胞中转染和毒性的研究 |
| Study on Transfection and Cytotoxicity of HydrophobicallyModifiedCationic Polymer SP-Chol in COS-7 Cells |
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| DOI： |
| 中文关键词: 疏水修饰 精胺 聚阳离子高分子 转染 毒性 |
| 英文关键词: Hydrophobically modified Spermine Cationic Polymer Transfection Cytotoxicity |
| 基金项目:国家" 重大新药创制" 科技专项（2009ZX09310-007：针对生物技术药物的相关给药技术平台） |
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| 中文摘要: |
| 目的：研究以精胺为单体，以乙二醇二氯甲酸酯作为连接剂，以胆固醇氯甲酸酯作为疏水基团连接剂合成的疏水修饰聚阳
离子高分子SP-Chol 对非洲绿猴肾癌细胞COS-7 的转染活性和细胞毒性的影响。方法：以荧光素酶质粒为报告基因，研究
SP-Chol 与DNA 的复合物在COS-7 细胞的转染活性，用MTT 方法研究SP-Chol 对COS-7 细胞的毒性。结果：COS-7 细胞实验显
示，SP-Chol 具有低于PEI 25kDa 的细胞毒性，同时也具有高效输送DNA 的能力。结论：SP-Chol 是一种新型的高效、低毒，在基因
治疗领域有潜在应用价值的非病毒基因输送载体。 |
| 英文摘要: |
| Objective: To investigate the transfection efficiency and cell cytotoxicity of hydrophobically modified cationic polymer
SP-Chol which is systhesized by using spermine as monomer, ethylenebis (chloroformate) as coupling agent and cholesterol chloroformate
as hydrophobic coupling agent. Methods: Luciferase plasmid was used as the reporter gene to investigate transfection efficiency on
COS-7 cells. Cytotoxicity was detected by MTT method. Results: COS-7 cells transfection and cytotoxicity results suggested that
SP-Chol was less cytotoxic than PEI 25kDa, and had a high transfection efficiency. Conclusion: SP-Chol is a highly efficient and less toxic
non-viral gene delivery vector which has potential application in gene therapy. |
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