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目的：探讨番茄红素对心肌细胞缺氧复氧的保护作用以及其分子机制。方法：采用原代培养心肌细胞建立缺氧/ 复氧损伤模型，实验分8 组：正常对照组，H/R 组，H/R+ 番茄红素(1,2,4,8,16,32 μmol/L)剂量组。观察各组细胞经H/R 损伤后，细胞内天冬氨酸氨基转移酶(AST)、肌酸激酶(CK)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的变化情况，选择正常对照组，H/R 组，最佳番茄红素剂量组做MTT 分析细胞凋亡，Western 检测TRL 4 以及NF-κB 的表达。结果：番茄红素(16,8,4,2 μmol/L)剂量组可显著降低缺氧/ 复氧损伤心肌细胞内AST、CK、LDH 释放量及MDA 的生成，并能提高SOD 活性。此外番茄红素可减少心肌细胞缺氧/ 复氧损伤后的心肌凋亡，减少TRL 4 受体以及NF-κB 的表达。结论：番茄红素具有抗缺氧/ 复氧损伤，保护心肌细胞的作用，其机制可能是通过抑制TRL 4 通路来实现的。

英文摘要:

Objective: To investigate the protective effects of Lycopene on neonatal rat cardiomyocytes injury induced by hypoxia/ reoxygenation (H/R)and to explore the mechanism. Methods: The H/R injury model of primary cultured neonatal rat cardiomyocytes was established. The cultured cardiomyocytes were divided into eight groups: normal control group, H/R group, H/R+Lycopene (1,2,4,8,16,32 μmol/L) group. The activities of aspartate aminotranferase(AST), creatine kinase(CK), lactate dehydrogenase(LDH), superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were determined. The numbers of apoptotic cardiomyocyte was determined by MTT, The expression of TLR-4 protein and NF-κB were detected by Western blot. Results: Lycopene groups(16,8,4,2 μmol/L) decreased the activities of AST, CK, LDH and the production of MDA, and significantly increased the activity of SOD. Compared with H/R group, Cardiomyocyte apoptosis in Lycopene group was significantly lower than that in H/R group but it was higher than that in the normal control group (P< 0.05). The expression of TLR- 4 protein and NF-κB protein in Lycopene group was significantly 1ower than that in the H/R group but it was higher than that in the normal control group.(P< 0.05). Conclusion: Lycopene has the effect of anti-hypoxia / reoxygenation injury and protection of myocardial cells, which may be achieved by inhibiting the pathway to TRL4.

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