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目的：研究人参皂甙Rd（Ginsenoside-Rd，GS-Rd）在大鼠局灶性脑缺血后对炎症趋化因子CXCL1 和γ- 干扰素（Interferon-γ, IFN-γ）的影响。方法：将SD 大鼠随机分为5 组：正常组（n=5），假手术组（n=5），GS-Rd 对照组（n=5），大脑中动脉栓塞模型（MCAO）组（n=20），MCAO+GS-Rd 组（n=20）。正常组不做任何处理；假手术组进行大脑中动脉栓塞手术，但不插入栓线；GS-Rd 对照组给予腹腔注射10 mg/Kg GS-Rd，不进行手术；MCAO 组（n=20）和MCAO+ GS-Rd 组（n=20）进行大脑中动脉栓塞手术，术后2 小时拔出栓线，MCAO+ GS-Rd 组在术前15 分钟腹腔注射10 mg/Kg GS-Rd。在12 小时、1 天、3 天、7 天四个时间点分别提取脑组织蛋白，通过液相芯片技术检测CXCL1，IFN-γ 含量。结果：正常组，假手术组和GS-Rd 对照组组间CXCL1，IFN-γ 含量无统计学差异；与三个对照组相比，MCAO 组和MCAO+ GS-Rd 组中CXCL1，IFN-γ蛋白含量均有明显增加（P<0.05）；而与MCAO 组相比，MCAO+ GS-Rd 组CXCL1，IFN-γ的生成明显减少（P<0.05）。结论：10 mg/Kg GS-Rd 预处理可有效抑制大鼠短暂性脑缺血后CXCL1，IFN-γ的生成；通过抑制炎症反应，GS-Rd 可能在神经保护中发挥重要的作用。

英文摘要:

Objective: To investigate the effect of Ginsenoside-Rd (GS-Rd) on protein expression of CXCL1 and IFN-γ(interferon- γ, IFN-γ)in rats after focal cerebral ischemia. Methods: A total of 55 rats were randomly divided into the five groups: blank group (n=5); Sham group (n=5),with surgery but no occlusion; GS-Rd group (n=5),with 10 mg/kg GS-Rd treatment but no surgery; middle cerebral artery occlusion(MCAO) group (n=20),with vehicle application before surgery; MCAO+GS-Rd group (n=20),with 10 mg/kg GS-Rd treatment before MCAO. After 2 h of occlusion, the suture was carefully removed to restore blood flow. Then the brain protein were extracted respectively at 12hour, 1day, 3day, 7day and were tested by LUMINEX 200. Results: Our results showed that the protein levels of CXCL1 and IFN-γwere significantly increased afterMCAO insult at all time points （p<0.05）. Pretreatment of 10 ml/kg GS-Rd inhibited MCAO-induced protein expressions of CXCL1 and IFN-γ（p<0.05）. Conclusion: These results suggest that neuroprotection of GS-Rd following cerebral ischemia may be at least due to inhibition of the proteins expression of CXCL1 and IFN-γ.

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