| 廖秋菊王晶△ 秦俭王长远田欣.腹腔注射法制备一氧化碳中毒迟发性脑病大鼠模型[J].,2011,11(6):1033-1036 |
| 腹腔注射法制备一氧化碳中毒迟发性脑病大鼠模型 |
| Preparation of carbon monoxide poisoning delayed encephalopathyrat model by intraperitoneal injection |
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| DOI： |
| 中文关键词: 急性一氧化碳中毒 迟发性脑病 动物模型 大鼠 |
| 英文关键词: Acute carbon monoxide poisoning Delayed neuropsychologic sequelae Animal mode Rat |
| 基金项目: |
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| 中文摘要: |
| 目的：建立可靠的急性一氧化碳(CO)中毒迟发性脑病的动物模型。方法：雄性SD 大鼠,分次腹腔注射CO 染毒制备模型,动
态监测尾血碳氧血红蛋白(HbCO)浓度；Morris 水迷宫检测大鼠1-5w 逃避潜伏期；尼氏染色及TUNEL 原位末端凋亡染色检测大
脑皮质及海马细胞损伤及凋亡。结果：染毒后,大鼠出现典型的CO 重度中毒症状，体内血液HbCO 浓度迅速升高,使用分次腹腔
注射法,大鼠可维持长时间(>12h)高HbCO 状态(HbCO>48%)；中毒组大鼠水迷宫检测认知功能较对照组下降，病理学检查显示大
鼠出现脑细胞损伤、凋亡明显。结论：本研究建立了一种较为符合迟发性脑病临床特征的动物模型,具有简单、可靠、重复性好的特
点，为深入研究急性CO 中毒致迟发性脑损伤的机制提供可靠基础。 |
| 英文摘要: |
| Objective: To establish a convenient and reliable delayed neuropsychologic sequelae rat model with acute carbon
monoxide (CO) poisoning. Methods: Male SD rats were injected CO repeatedly, then dynamically monitored the carboxyhemoglobin
(HbCO) level in blood for ascertaining the administration dose and process; Cognitive ability was measured by the escape latency of
searching the objective in the Morris watermaze; Neuronal injury were detected by Nissl stain, TUNEL stain was used to assess apoptosis.
Results: The HbCO levels rised rapidly,and kept at higher level (>48%) for long time (>12h) by the method of repeated injection of CO
ip; The cognitive ability in CO group was lower than that of the control group; The pathological examination revealed apparent neuronal
degeneration and necrosis in the cerebral in CO poisoned rats. Conclusion: CO peritoneum injection was aeffective, convenient, and reliable,
which offered reliable scientific basis for further exploring the pathogenesis of DNS by CO. |
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