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目的：分析人肝癌（HCC）组织中染色体8p、16q 部分基因及染色体片段的遗传变异及与临床病理关系，初步筛选HCC 相关的抑癌基因。方法：应用聚合酶链反应- 变性聚丙烯酰胺凝胶- 银染法分析45 例HCC 组织标本中染色体8p 和16q 的杂合性丢失（LOH）及微卫星不稳定性（MSI）。结果：发生LOH 的总频率为68.89%(31/45)，其中D16S511 位点的发生LOH 率最高为 53.33%(24/45)，其次是D8S261（39.02%，16/41）和D8S499（34.88%，15/43）。MSI 出现的总频率为11.11%（5/45），出现在三个微卫星位点（D8S261、D8S499 及D16S511）上。结论：染色体16q23、8p22-21.3 及8p12 区域的LOH 发生频率高，其可能存在与HCC 发生发展相关的新的抑癌基因，特定位点的遗传变异可能与HBV 感染、临床病理恶性程度等预后因素相关。

英文摘要:

Objective: To investigate the genetical alterations on chromosomes 8p and 16q in primary hepatocellular carcinoma (HCC), investigate the relationship between the gentical alterations and clinicopathologic features and try to screened some HCC-related tumor suppressor genes. Methods: Loss of heterozygosity (LOH) and microsatellite instability (MSI) on chromosome 8p and 16q in samples from thirty-five patients with HCC were examined by PCR-denaturing PAGE-silver staining. Results: The overall LOH frequency was 68.89%(31/45) at least one locus of 8 loci on the chromosomes. The frequency of Loci were 53.33% (31/45),39.02% （16/41）and34.88%（15/43）for D16S511, D8S261 and D8S499, respectively. The frequency of MSI was 11.11%(5/45) and the MSI was distributed on the three microsatellite markers (D16S511、D8S261 and D8S499). Conclusion: There may be a new putative tumor suppressor gene related to the occurrence and development on specific chromosome region 16q23, 8p22-21.3 or 8p12 with high-frequent LOH. The genetic alterations on some specific loci were associated with prognosis factors as the positive HBsAg, differentiated degrees of HCC.

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