| 谌海燕1 侯丽1 陈信义2.耐三苯氧胺乳腺癌异种移植瘤动物模型建立与评价[J].,2011,11(5):841-843 |
| 耐三苯氧胺乳腺癌异种移植瘤动物模型建立与评价 |
| Establishment of xenograft model in nude mice oftamoxifen -resistanthuman breast cancer |
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| DOI： |
| 中文关键词: 乳腺癌 移植瘤 三苯氧胺 |
| 英文关键词: Breast Cancer Xenograft Tamoxifen |
| 基金项目:北京中医药大学自主创新课题(2009TYBZZ-XS057) |
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| 摘要点击次数: 899 |
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| 中文摘要: |
| 目的：建立耐三苯氧胺（TAM）人乳腺癌的裸鼠移植瘤模型，为研究和治疗乳腺癌对TAM 耐药提供研究工具。方法：采用雌
激素受体阳性，对TAM 耐药的人乳腺癌细胞系LCC2，接种于BALB/c 裸鼠皮下，观察肿瘤生长趋势，用免疫组化方法进行鉴定。
结果：在接种细胞数大于5×106/ 只时，Matrigel 能够显著促进移植瘤的生长。肿瘤组织病理学检测证实为浸润性导管癌，且Pgp
和Her-2 为阳性表达。结论：该方法建立的耐TAM 人乳腺癌移植瘤模型，周期短，成瘤率高，保留了与细胞系相同的肿瘤生物学
特征。 |
| 英文摘要: |
| Objective: This study aims to establish the xenograft model of human breast cancer resistant to tamoxifen (TAM)so as
to provide a research tool for the study and treatment of breast cancer resistant to TAM.Methods: This study employs the method of
inoculating the human breast cancer cells LCC2 which is estrogen receptor positive and resistant to TAM into BALB/c nude mice.Then it
observes the trends of tumor growth,and identifies by immunohistochemistry methods. Results: When the number of inoculation cells is
more than 5×106,Matrigel can significantly promote tumor growth.Pathology confirms that the xenograft is invasive ductal carcinoma,
and Pgp,Her-2 are positively expressed.Conclusions: The method of establishing the xenograft model of human breast cancer resistant to
TAM has short cycle,high rate of tumor formation, and retains the same characteristics of tumor biology with cell lines.
Key words: Breast Cancer;Xenograft;Tamoxifen |
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