| 莫镜崔其亮△ 张费通林黎黎李颖廖嘉仪.钙离子浓度动态变化[J].,2011,11(5):834-836 |
| 钙离子浓度动态变化 |
| Detection of Dynamic Changes of Intracellular Calcium in HypoxiaHuman Pulmonary Microvascular Endothelial Cellsby Laser Scanning Confocal Microscope |
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| DOI： |
| 中文关键词: 激光扫描共聚焦显微镜 缺氧 人肺微血管内皮细胞 钙离子浓度 |
| 英文关键词: Laser scanning confocal microscope Hypoxia Human pulmonary microvascular endothelial cells Intracellular free
calcium concentration. |
| 基金项目:广东省科技计划项目（粤科规划字[2010]170 号） |
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| 中文摘要: |
| 目的：探讨应用激光共聚焦扫描显微镜（LSCM）技术检测缺氧状态的人肺微血管内皮细胞（HPMVEC）内钙离子（Ca2+）浓度
动态变化的价值。方法：HPMVEC 常规培养，按观察时间点不同分为5 个缺氧培养组（1h hyp 组、2h hyp 组、4h hyp 组、6h hyp 组
和8h hyp 组）以及1 个对照组（0h con 组）共6 个组，每组设8 个复孔，应用LSCM 技术测定缺氧后HPMVEC 内Ca2+ 浓度水平及
随时间推移的变化。结果：LSCM 技术显示HPMVEC 内Ca2+ 的荧光强度1h hyp 组与0h con 组比较、2h hyp 组与1h hyp 组比
较、4h hyp 组与2h hyp 组比较、6h hyp 组与4h hyp 组比较、8h hyp 组与6h hyp 组比较有显著差异（P<0.05）。线性回归分析结果显
示Ca2+ 荧光强度与缺氧时间成正相关（r=0.969，P<0.01）。结论：HPMVEC 内Ca2+ 浓度随缺氧时间增长而增高；LSCM 在动态检
测缺氧状态下HPMVEC 内的Ca2+ 浓度变化中具有明显优势。 |
| 英文摘要: |
| Objective: To investigate the dynamic changes of intracellular calcium concentration ([Ca2+]i) by laser scanning
confocal microscope (LSCM) in hypoxia human pulmonary microvascular endothelial cells (HPMVEC). Methods: HPMVEC
conventional culture, divided into six groups: one control group ( Group 0h con) and five hypoxia groups，respectively hypoxia for 1h,
2h, 4h, 6h and 8h(1h hyp group, 2h hyp group, 4h hyp group, 6h hyp group and 8h hyp group),each group has 8 wells. The changes of the
intracellular calcium concentration ([Ca2+]i) were determined after hypoxia by LSCM. Results: The concentration of the intracellular
calcium was significantly increased in the 1h hyp group compared to the 0h hyp group(P<0.05), and in the 2h hyp group compared to the
1h hyp group(P<0.05), and in the 4h hyp group compared to the 2h hyp group(P<0.05), and in the 6h hyp group compared to the 4h hyp
group(P<0.05), and in the 8h hyp group compared to the 6h hyp group(P<0.05). Linear regression showed: Fluorescence intensity of the
intracellular calcium concentration positively correlated with hypoxia (r=0.969，p<0.01). Conclusions: The intracellular calcium
concentration of hypoxia HPMVEC were increasing with the time. It is an obvious advantages that LSCM dynamicly detects the change
of intracellular calcium concentration of the HPMVEC. |
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