刘博苏文静马力潘蕾连建奇黄长形白雪帆△.大剂量HBsAg 对HBV 转基因小鼠T 细胞免疫调节的研究[J].,2011,11(5):812-814 |
大剂量HBsAg 对HBV 转基因小鼠T 细胞免疫调节的研究 |
Study on T Cellular Immune Response to Large-dose HBsAg inTransgenic Mice |
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DOI: |
中文关键词: 肝炎病毒 乙型 HBsAg 转基因小鼠 免疫耐受 |
英文关键词: Hepatitis B virus HBsAg Transgenic mice Immune tolerance |
基金项目:国家科技重大专项资助项目(2008ZX10204) |
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中文摘要: |
目的:研究大剂量HBsAg 对HBV 转基因小鼠其T 细胞免疫效果的影响。方法:用大剂量血源性HBsAg 免疫HBV 转基因
小鼠, 采用ELISA 方法观察转基因小鼠所诱生的HBsAg 特异性Th1 类细胞因子的水平,ELISPOT 方法检测不同免疫方案对小
鼠HBsAg 特异性分泌IFN-γT 细胞数量的影响,同时检测对小鼠淋巴细胞增殖的影响。结果:HBsAg 组免疫后脾细胞产生的Th1
类细胞因子(IFN-γ、IL-2)、HBsAg 特异性分泌IFN-γT 细胞及T 细胞增殖水平较对照组显著增加(P<0.05)。结论:大剂量的HBs-
Ag 可以诱导乙肝转基因小鼠产生高水平Th1 类细胞因子并打破免疫耐受。 |
英文摘要: |
Objective: To investigate the effect of HBsAg on T cellular immune responses in HBV transgenic mice. Methods:
HBV transgenic mice were immunized by HBsAg and Sodium Chloride. The Th1 cytokines (IFN-γ, IL-2) were detected by ELISA and
the number of IFN-secreting T cells was examined by ELISPOT. The proliferation of spleen cells was measured by MTT. Rsults:
Compared with control group, the IL-2,IFN-γ and HBs-specific IFN-secreting T cell of HBsAg group increased, and strong lymphocyte
proliferation reaction was induced (p<0.05). Conclusion: HBsAg could increase Th1 cytokines in transgenic mice and break the
immune tolerance to antigen in HBV transgenic mice. |
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