| 牛培广1 张钰璇2 史道华2.己糖激酶介导小豆蔻明改善血管平滑肌细胞糖代谢的作用[J].,2011,11(4):653-656 |
| 己糖激酶介导小豆蔻明改善血管平滑肌细胞糖代谢的作用 |
| Improvement Effects of Cardamonin on Glucose Metabolism ofVascular Smooth Muscle Cells Induced by Hexokinase |
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| DOI： |
| 中文关键词: 小豆蔻明 胰岛素抵抗 糖消耗 己糖激酶 |
| 英文关键词: Cardamonin Insulin Resistance Glucose Consumption Hexokinase |
| 基金项目:福建省自然科学基金项目（2008J0103） |
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| 中文摘要: |
| 目的：探讨小豆蔻明对胰岛素抵抗状态（IR）血管平滑肌细胞（VSMCs）糖代谢的影响及其作用机制。方法：采用高糖( 2.5×
l0-2 mol·L-1)高胰岛素( 100 U·L-1 )培养VSMCs，72 h 后加入小豆蔻明培养48 h，观察培养液中葡萄糖消耗量、细胞内己糖激酶活
性以及细胞增殖。结果：高糖高胰岛素培养72 h后，血管平滑肌细胞糖消耗量降低（P<0.01）；小豆蔻明能显著性增加IR细胞的平
均糖消耗量（P<0.01），增强己糖激酶活性（P<0.01）；同时明显抑制高糖高胰岛素引起的VSMCs 增殖（P<0.01）。结论：小豆蔻明能
增强己糖激酶活性，改善IR状态下细胞糖代谢；同时抑制高糖高胰岛素刺激引起的VSMCs异常增殖。 |
| 英文摘要: |
| Objective: To investigate the effects of cardamonin on regulation of glucose metabolism on the vascular smooth muscle
cells (VSMCs) and its possible mechanism in the insulin-resistant condition. Methods: VSMCs were cultured in high glucose ( 2.5×
l0-2 mol·L-1) and high insulin (100 U·L-1) medium for 72 hours, then cardamonin was added to incubate for 48 hours. Glucose consumption,
hexokinase activity and proliferation of VSMCs were measured in the experiment. Results: Cultured with high glucose and high insulin
for 72 hours, glucose consumption of VSMCs was significantly decreased (P<0.01). Cardamonin significantly increased the glucose
consumption and hexokinase activity of VSMCs in IR statue (P<0.01) and inhibited the proliferation induced by high glucose and high
insulin (P<0.01). Conclusion: The results suggested that cardamonin could improve the glucose metabolism and inhibit the proliferation
of VSMCs in the IR condition, which might be related to enhancement of hexokinase activity. |
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