| 贾济1 朱萧玲1 左志义2 熊利泽1 陈绍洋1.CBR2激活与小胶质细胞的活化和损伤的关系[J].,2011,11(4):601-604 |
| CBR2激活与小胶质细胞的活化和损伤的关系 |
| The relationship between CBR2 Activation and Injury Inducedby Inflammation |
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| DOI： |
| 中文关键词: CBR2 小胶质细胞 炎症 活化 损伤 |
| 英文关键词: CB2 Microglia Inflammation Activation Injury |
| 基金项目:国家自然科学基金资助课题（81050032）；海外及港澳学者合作研究基金（81028006） |
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| 中文摘要: |
| 目的：探讨大麻素CBR2 受体激动剂AM1241预处理对脂多糖(LPS)和γ-干扰素(IFN-γ)所致炎症反应对小胶质细胞活化
和损伤的影响。方法：联用LPS 和IFN-γ 作为小胶质细胞损伤模型，将细胞分为Control 组、AM1241 组、LPS/IFN-γ 组和
AM1241+ LPS/IFN-γ 组；AM1241 组和AM1241+ LPS/IFN-γ 组经AM1241 预处理2h，LPS/IFN-γ 组和AM1241+ LPS/IFN-γ
组用含LPS和IFN-γ 的培养基培养24h。采用MTT法检测细胞代谢率，硝酸还原酶法检测细胞培养液中一氧化氮（NO）释放量，
酶联免疫吸附剂测定细胞培养基中炎症因子释放量，倒置相差显微镜观察细胞形态。结果：与LPS/IFN-γ 组相比，AM1241+
LPS/IFN-γ 组细胞代谢率明显升高（P＜0.05），NO、TNF-α、IL-1β 和IL-10 释放量明显减少（P＜0.05），活化和损伤程度明显减
轻。结论：大麻素CBR2 受体激动剂AM1241预处理可减轻LPS和IFN-γ 对小胶质细胞的活化和损伤。 |
| 英文摘要: |
| Objective: To investigate the effects of cannabinoid CBR2 receptor agonist AM1241 preconditioning on microglia
activation and injury induced by lipopolysaccharide (LPS) plus interferon-γ (IFN-γ). Methods: LPS plus IFN-γ was used to induce
inflammation. MTT assay was used to find a suitable AM1241 concentration for preconditioning. Cells were pretreated with medium
containing different AM1241 concentrations, from 0μM to 10μM; 5μM was chosen for next steps. Then cells were assigned to control
group, AM1241 group, LPS/IFN-γ group and AM1241+LPS/IFN-γ group. After AM1241 pretreatment, the medium of 4 groups were
changed with normal medium. 2h later, the medium of Control and AM1241 groups were changed with normal medium again and cultured
for 24h. The medium of LPS/IFN-γ and AM1241+ LPS/IFN-γ groups were changed with medium containing LPS and IFN-γ.
Cells were cultured for 24h. Microglial metabolism was assessed by MTT assay; NO release was measured by Reagent Kit; The concentrations
of inflammatory factors (TNF-α, IL-1β and IL-10) were detected by enzyme linked immunosorbent assay reagent kit (ELISA);
Microglial shapes were observed through microscope. Results: cell metabolism of AM1241 group was higher than that of the Control
group significantly (P＜0.05); AM1241 group released less NO, TNF-α, IL-1β and IL-10 than that of LPS/IFN-γ group (P＜0.05).
Conclusion: Cannabinoid CBR2 receptor agonist AM1241 preconditioning reduces microglial activation and injury induced by inflammation. |
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