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目的：应用牛血清白蛋白荧光猝灭法建立一种测定槐花中芦丁含量的新方法。方法：牛血清白蛋白（BSA）具有很强的内源荧光性，而芦丁溶液本身不产生荧光。当芦丁与BSA结合后，会导致其荧光强度下降，表面活性剂吐温-80（T-80）对体系有促进荧光猝灭作用。BSA在λex=338 nm处的荧光猝灭程度与芦丁的量在一定浓度范围内呈良好的线性关系，据此建立测定槐花中芦丁含量的新方法。结果：该结合物的最大发射波长为λmax=338nm，与芦丁摩尔浓度在6×10-7~3.0×10-5 mol·L-1范围内线性关系良好。其线性回归方程为ΔF=136.36 CRu(×10-5 mol·L-1)-0.5454，相关系数r =0.9976，检出限为1.58×10-7 mol·L-1，RSD 为2.8%~4. 3%，加标回收率为97.6%~101.2%。结论：本方法操作简便、快速，用于实际样本的测定，结果满意。

英文摘要:

Objective: To investigate a new method to decte the rutin in flos sophorae by fluorescence quenching of bovine serum albumin. Methods: The Bovine serum albumin (BSA) has a strong intrinsic fluorescence because of tryptophan and tyrosine. The fluorescebce intensity of BSA decreased when the rutin combination with the BSA, and surface-active agent tween -80 (T-80) inhanced the fluorescence quenching on the system. In a certain range, the fluorescence quenching of BSA in the λex = 338 nm has a good linear relationship with the concentration of rutin. The new method to detect the rutin in flos sophoraes was established. Results: The conjugate of BAS and rutin had a max fluorescebec value at 338 nm which was found the decreased intensity of fluorescence at 338nm was proportion to rutin in the range of 6×10-7~3.0×10-5 mol·L-1.The linear regression equation was ΔF=136.36 CRu(×10-5 mol·L-1)-0.5454 with a correlation coefficient of 0.9976. The detection limit was 1.58×10-7 mol·L-1. The relative standard deviation was 2.8%~4.3% and the average recovery of sample was 97.6%~101.2%. Conclusion: The method was simple and rapid. It can be applied to detect the trace rutin in flos sophorae with satisfactory results.

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