| 张小林1 韩丽君2 李龙2 林志雄1△.RNA甲醛琼脂糖凝胶电泳的优化及探讨[J].,2011,11(2):351-353 |
| RNA甲醛琼脂糖凝胶电泳的优化及探讨 |
| The Opitimization and Discussion of RNA Formaldehyde AgaroseGel Electrophoresis* |
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| DOI： |
| 中文关键词: RNA RNase 电泳 琼脂糖 |
| 英文关键词: RNA RNase electrophoresis agarose |
| 基金项目:国家自然科学基金资助（30973083） |
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| 中文摘要: |
| 目的：优化RNA甲醛琼脂糖凝胶电泳条件。方法：对实验器材进行彻底的RNase 灭活处理;在原有电泳过程的基础上,增加
预电泳、缓冲液液面的处理和制胶过程中未加入溴化乙锭等优化过程。结果：优化后的电泳不仅能验证总RNA 的完整性,而且能
鉴定RNA的分子大小。结论：优化的RNA甲醛琼脂糖凝胶电泳法操作简单、省时、快速,RNA条带清晰、定位准确、无弥散及非特
异条带。 |
| 英文摘要: |
| Objective: To opitimize the conditions of the RNA formaldehyde agarose gel electrophoresis. Methods: Thoroughly inactivate
RNase of experimental equipments; Based on the original electrophoresis process,adding the opitimized procedures, such as:
pre-electrophoresis, treatment of buffer surface and decreasing ethidium bromide into gel etc. Results: The opitimized RNA electrophoresis
not only verify the integrity of total RNA, but also identify the molecular size of RNA. Conclusion: The opitimized RNA electrophoresis
is simple, time-saving, fast and RNA bands are clear, accurate position, no dispersion and non-specific bands. |
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