齐凤琴李廷利△ 张冰张博郭冷秋黄莉莉.RP-HPLC 法测定酸枣仁汤中甘草苷的含量[J].,2011,11(13):2543-2545 |
RP-HPLC 法测定酸枣仁汤中甘草苷的含量 |
Determination of Liquiritin in Suanzaoren Decoction by RP-HPLC |
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DOI: |
中文关键词: 酸枣仁汤 芒果苷 RP-HPLC |
英文关键词: Suanzaoren Decoction Liquiritin RP-HPLC |
基金项目:2008 年教育部博士点基金(200802280001) |
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中文摘要: |
目的:建立测定酸枣仁汤中甘草苷含量的反相高效液相色谱分析方法。方法:色谱柱:WatersC18 (250nm×4.6mm,5μm);流
动相:乙腈-0.1%磷酸水溶液,梯度洗脱;流速:1.0ml/min,检测波长:276nm。结果:芒果苷在0.093μg-0.744μg 范围内与峰面积线
性关系良好(r=0.9999),平均加样回收率为98.04%,RSD 为0.41%。结论:本方法简便、准确、可靠、重复性好,可作为酸枣仁汤中
甘草苷的含量测定方法。 |
英文摘要: |
Objective: To establish a HPLC method for the determination of Liquiritin in Suanzaoren Decoction. Methods:The
determination was performed on WatersC18 column (250nm×4.6mm,5μm)with a grient mobile phase composed of acetionitrile and
0.1% phosphoric acid. The detection wavelength was 276nm,and the flow rate was 1ml/min.Results:The Mangiferin showed good
linearity (r=0.9999)in the range of 0.093μg-0.744μg, the average recovery was 98.04% with RSD 0.41%. Conclusions:The method is
simple,accurate,reliable and is suitable for Determination of Liquiritin in Suanzaoren Decoction. |
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