赵彦禹 张艳华 冯照军.鸡红细胞融合最适条件的探讨[J].,2006,6(1):43-44 |
鸡红细胞融合最适条件的探讨 |
Best Condition Studies on Fusion of Chicken Red Blood Cells |
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DOI: |
中文关键词: 细胞融合 鸡红细胞 聚乙二醇 |
英文关键词: cell fusion chicken red blood cells PEG |
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中文摘要: |
目的: 探讨快速、有效的细胞融合条件。方法: 用鸡红细胞为材料, 聚乙二醇(Mw = 4000) 为诱导剂, 诱导鸡红细胞融
合。结果: 鸡红细胞融合的最适温度为39 ?? , 最适时间为15min。结论: 在该条件下, 同时用Giemsa 染液对融合细胞染色, 实验观
察效果明显。 |
英文摘要: |
Objective: to find a more effective and easier method of cell fusion. Methods: Make chicken red blood cells to fusion, which
was induced by PEG (Mw = 4000) . Results: the most appropriate condition for cell fusion in the chicken red blood cells is 39 centigrade
and15minutes. Conclusion: on this condition, the fusion phenomenon of chicken red blood cells is evident. |
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