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| 支气管哮喘患者血清miR-29a-3p、miR-98-5p表达水平与肺功能、气道炎症和糖皮质激素治疗敏感性的关系研究* |
| Relationship Study Between Serum miR-29a-3p and miR-98-5p Expression Levels and Lung Function, Airway Inflammation and Sensitivity to Glucocorticoid Therapy in Patients With Bronchial Asthma |
| 投稿时间：2023-03-13 修订日期：2023-03-13 |
| DOI： |
| 中文关键词: 支气管哮喘 miR-29a-3p miR-98-5p 肺功能 气道炎症 糖皮质激素 敏感性 |
| 英文关键词: Bronchial asthma microRNA-29a-3p microRNA-98-5p Lung function Airway inflammation Glucocorticoid Sensitivity |
| 基金项目:山东省医药卫生科技发展计划项目（2017WS116） |
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| 中文摘要: |
| 目的 探讨支气管哮喘（BA）患者血清微小核糖核酸（miR）-29a-3p、miR-98-5p表达水平与肺功能、气道炎症和糖皮质激素（GC）治疗敏感性的关系。方法 选取2020年1月～2022年1月潍坊市人民医院收治的150例BA患者为BA组，根据BA患者GC治疗敏感性将其分为抵抗组43例和敏感组107例，另选取同期57名体检健康者为对照组。收集BA组、对照组肺功能和气道炎症指标资料，采用实时荧光定量逆转录聚合酶链式反应（qRT-PCR）检测两组血清miR-29a-3p、miR-98-5p表达水平。通过Spearman相关性分析BA患者血清miR-29a-3p、miR-98-5p表达水平与肺功能和气道炎症指标的相关性，单因素和多因素Logistic回归分析BA患者GC治疗抵抗的影响因素。结果 与对照组比较，BA组血清miR-29a-3p、miR-98-5p表达水平和第1秒用力呼气容积占预计值百分比（FEV1％）、第1秒用力呼气容积/用力肺活量（FEV1/FVC）、峰值呼气流速（PEF）降低，呼出气一氧化氮（FeNO）水平升高（P均＜0.05）。Spearman相关性分析显示，BA患者血清miR-29a-3p、miR-98-5p表达水平与FEV1％、FEV1/FVC、PEF呈正相关，与FeNO水平呈负相关（P均＜0.05）。单因素分析显示，抵抗组体质指数＞24kg/m2、吸烟比例高于敏感组，血清miR-29a-3p、miR-98-5p表达水平低于敏感组（P＜0.05）。多因素Logistic回归分析显示，体质指数＞24kg/m2、吸烟为BA患者GC治疗抵抗的独立危险因素，血清miR-29a-3p、miR-98-5p表达水平升高为其独立保护因素（P均＜0.05）。结论 BA患者血清miR-29a-3p、miR-98-5p水平降低，与肺功能下降、气道炎症和GC治疗抵抗有关。 |
| 英文摘要: |
| Objective To investigate the relationship between serum micro ribonucleic acid (miR)-29a-3p and miR-98-5p expression levels and lung function, airway inflammation and sensitivity to glucocorticoid (GC) therapy in patients with bronchial asthma (BA). Methods 150 patients with BA who were admitted to Weifang People''s Hospital from January 2020 to January 2022 were selected as BA group. According to sensitivity to GC therapy of patients with BA, they were divided into resistance group with 43 cases and sensitive group with 107 cases, and 57 healthy subjects during the same period were selected as control group. The data of lung function and airway inflammation indexes in the BA group and control group were collected, and the serum miR-29a-3p and miR-98-5p expression levels in the two groups were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction (qRT-PCR). Spearman correlation was used to analyze the correlation between the serum miR-29a-3p and miR-98-5p expression levels in patients with BA and lung function and airway inflammation indexes, and univariate and multivariate Logistic regression were used to analyze the influencing factors of GC resistance in patients with BA. Results Compared with the control group, the serum miR-29a-3p and miR-98-5p expression levels, the percentage of forced expiratory volume in the first second to the predicted value (FEV1%), forced expiratory volume in one second/forced vital capacity (FEV1/FVC) and peak expiratory flow (PEF) in the BA group were decreased, the fractional exhaled nitric oxide (FeNO) level was increased (all P<0.05). Spearman correlation analysis showed that the serum miR-29a-3p and miR-98-5p expression levels in patients with BA were positively correlated with FEV1%, FEV1/FVC and PEF (all P<0.05), and negatively correlated with FeNO level (all P<0.05). Univariate analysis showed that the body mass index>24kg/m2, smoking ratio in the resistance group were higher than those in the sensitive group, and the serum miR-29a-3p and miR-98-5p expression levels were lower than those in the sensitive group (P<0.05). Multivariate Logistic regression analysis showed that body mass index>24kg/m2, smoking were independent risk factors for GC resistance in patients with BA, and increased serum miR-29a-3p and miR-98-5p expression levels were independent protective factors (all P<0.05). Conclusions Serum miR-29a-3p and miR-98-5p levels decreased in patients with BA, which are associated with decreased lung function, airway inflammation and resistance to GC therapy. |
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