| Objective: To explore the relationship between the serum renin angiotensin system related factors [angiotensin (Ang)Ⅱ, Ang- (1-7)] expression levels and ovarian interstitial blood flow in patients with polycystic ovary syndrome (PCOS). Methods: A total of 88 patients with PCOS who were diagnosed and treated in our hospital from July 2020 to October 2021 were selected, and the candidates were divided into two groups according to the insulin resistance index (HOMA-IR). Those with HOMA-IR ≥ 2.69 were divided into insulin resistance group (PCOS-IR group, n=42), and those with HOMA-IR < 2.69 were divided into non insulin resistance group (PCOS-NIR group, n=46). In addition, 90 healthy female volunteers who underwent physical examination in our hospital were selected as the control group. The serum Ang Ⅱ and Ang-(1-7) expression levels were measured by enzyme-linked immunosorbent assay (ELISA). Meanwhile, the ovarian interstitial blood flow indexes of all subjects were monitored by EPIQ5 color Doppler ultrasound on the day of blood collection, and the peak blood flow velocity (PSV), pulsatility index (PI) and resistance index (RI) were calculated. Pearson test was used to study the correlation between Ang Ⅱ, Ang- (1-7) and ovarian interstitial blood flow in patients with PCOS. Multivariate Logistic regression analysis was used to analyze the influencing factors of ovarian interstitial blood flow in patients with PCOS. Results: Compared with the control group, the levels of Ang Ⅱ, Ang-(1-7), PSV and HOMA-IR in PCOS group were significantly higher, while the levels of PI and RI were significantly lower (P<0.05). Compared with PCOS-NIR group, the levels of Ang Ⅱ, Ang-(1-7), PSV and HOMA-IR in PCOS-IR group were significantly higher, while the levels of PI and RI were significantly lower (P<0.05). Pearson correlation analysis showed that Ang Ⅱ and Ang-(1-7) were positively correlated with PSV and HOMA-IR (P<0.05), but negatively correlated with PI and RI (P<0.05). There was a positive correlation between Ang Ⅱ and Ang-(1-7) (P<0.05). Logistic multifactor regression analysis showed that Ang Ⅱ, Ang-(1-7) and HOMA-IR were related influencing factors of ovarian interstitial blood flow in patients with PCOS (P<0.05). Conclusion: Ang Ⅱ and Ang-(1-7) are highly expressed in the serum of patients with PCOS, and there is a positive correlation between them. The expression levels are further increased in the serum of patients with PCOS with insulin resistance. The imbalance of the two expressions play an important role in the increase of abnormal ovarian interstitial blood flow in patients with PCOS. |
