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| 肺纤维化患者血清MicroRNA let-7、MicroRNA-29表达及其临床意义 |
| Expression and Clinical Significance of Serum MicroRNA let-7 and MicroRNA-29 in Patients With Pulmonary Fibrosis |
| 投稿时间：2021-09-04 修订日期：2021-09-04 |
| DOI： |
| 中文关键词: 肺纤维化 MicroRNA let-7 MicroRNA-29 肺功能 诊断价值 |
| 英文关键词: Pulmonary fibrosis MicroRNA let-7 MicroRNA-29 Pulmonary function Diagnostic value |
| 基金项目:重庆市璧山区科研项目（bskj20200）；重庆市卫生局医学科研计划重点项目（2011-1-050） |
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| 中文摘要: |
| 目的 探讨肺纤维化患者血清MicroRNA let-7、MicroRNA-29表达及其临床意义。方法 收集2019年4月-2021年2月我院就诊的肺纤维化患者43例作为研究组，同时收集50例健康体检患者作为对照组。采用实时荧光定量PCR（qRT-PCR）检测各组血清MicroRNA let-7、MicroRNA-29表达水平。采用Pearson相关分析研究组患者血清MicroRNA let-7、MicroRNA-29的表达水平与炎症指标、肺功能的相关性。应用ROC曲线分析血清MicroRNA let-7、MicroRNA-29表达水平对肺纤维化患者的预测诊断价值。结果 研究组患者肺功能指标一秒用力呼气容积/用力肺活量（FEV1/FVC）、一氧化碳弥散量占预计值的百分比（DLco%pred）、动脉血氧分压（PaO2）均低于对照组，差异有统计学意义（P＜0.05）。研究组患者血清MicroRNA let-7、MicroRNA-29相对表达水平均显著低于对照组，差异有统计学意义（P＜0.05）。研究组患者血清MicroRNA let-7、MicroRNA-29的表达水平与FEV1/FVC、DLco%pred、PaO2均呈正相关（P＜0.05）。血清MicroRNA let-7、MicroRNA-29及两者联合对肺纤维化患者诊断效能的ROC曲线下面积（AUC）分别为0.743（95%CI:0.574-0.914），0.761（95%CI:0.597-0.926），0.848（95%CI:0.699-0.997）。血清MicroRNA let-7和MicroRNA-29联合应用对肺纤维化的诊断价值优于两者单一应用。结论 肺纤维化患者血清MicroRNA let-7、MicroRNA-29表达水平比正常人显著降低，且与肺功能指标呈正相关性，两者联合应用可提高预测诊断价值，可作为预测肺纤维化患者及其病情评估的生物学指标。 |
| 英文摘要: |
| Objective:To investigate the expression and clinical significance of serum MicroRNA let-7 and MicroRNA-29 in patients with pulmonary fibrosis.Methods:43 patients with pulmonary fibrosis treated in our hospital from April 2019 to February 2021 were collected as the study group, and 50 healthy patients were collected as the control group.The expression levels of serum MicroRNA let-7 and MicroRNA-29 were detected by real-time fluorescence quantitative PCR (qRT-PCR). Pearson correlation analysis was used to study the correlation between the expression levels of serum MicroRNA let-7 and MicroRNA-29 and inflammatory indexes and pulmonary function. ROC curve was used to analyze the predictive diagnostic value of serum MicroRNA let-7 and MicroRNA-29 expression levels in patients with pulmonary fibrosis.Results:The lung function indexes FEV1/FVC, DLco%pred and PaO2 of the study group were lower than those of the control group,and the difference was statistically significant (P＜0.05).The relative expression levels of serum MicroRNA let-7 and MicroRNA-29 in the study group were significantly lower than those in the control group,and the difference was statistically significant (P<0.05).The expression levels of serum MicroRNA let-7 and MicroRNA-29 in the study group were positively correlated with FEV1/FVC, DLco%pred, and PaO2 (P＜0.05).The area under the ROC curve (AUC) of serum MicroRNA let-7, MicroRNA-29 and the combination of the two in the diagnosis of pulmonary fibrosis patients were 0.743 (95%CI: 0.574-0.914) and 0.761 (95%CI: 0.597-0.926) ), 0.848 (95%CI: 0.699-0.997) . The combined application of serum MicroRNA let-7 and MicroRNA-29 has better diagnostic value for pulmonary fibrosis than a single application of the two.Conclusion:The expression levels of serum MicroRNA let-7 and MicroRNA-29 in patients with pulmonary fibrosis were significantly lower than those in normal subjects, and were positively correlated with lung function indexes. The combined application of the two can improve the predictive diagnostic value and can be used as biological indexes for predicting patients with pulmonary fibrosis and evaluating their condition. |
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