文章摘要
缺氧对椭圆囊毛细胞谷氨酸递质合成及释放的影响
Effects of Hypoxia on Synthesis and Release of Glutamate Acid in Utricle Hair Cells
投稿时间:2020-07-15  修订日期:2020-07-15
DOI:
中文关键词: 缺氧  椭圆囊  毛细胞  谷氨酸
英文关键词: Hypoxia  Utricle  Hair cells  glutamate acid
基金项目:军队实验动物专项科研课题(SYDW[2018]19号);空军军医大学航空航天医学系后备人才创新基金(2019HBRC12)
作者单位E-mail
张敏 空军军医大学航空航天医学系 zhangmin2015@fmmu.edu.cn 
李家卉 空军军医大学航空航天医学系  
刘玉辉 空军军医大学航空航天医学系  
李聪聪 空军军医大学航空航天医学系  
王小成△ 空军军医大学航空航天医学系 wxcnose@163.com 
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中文摘要:
      目的:探讨缺氧对豚鼠前庭耳石器椭圆囊毛细胞内神经递质谷氨酸合成及释放的影响。方法:分离4周龄豚鼠双侧椭圆囊进行体外培养,分别置于常氧(21% O2)和低氧(1% O2)环境下处理30 min,利用western blotting、酶活性检测等方法检测毛细胞中谷氨酰胺酶的活性和表达水平,评价谷氨酸合成变化情况;利用ELISA、免疫荧光等方法检测椭圆囊组织中谷氨酸含量和分布情况。结果:在给予缺氧处理后,豚鼠椭圆囊毛细胞中谷氨酸合成关键酶谷氨酰胺酶的表达、酶活性均未发生显著性变化;ELISA结果提示,相较于对照组,低氧组椭圆囊组织中谷氨酸水平降低5.6%(P<0.05);免疫荧光结果提示,相较于对照组,低氧组椭圆囊毛细胞内的谷氨酸更加广泛的分布于毛细胞基底部,而在纤毛一侧分布较少。结论:在体外培养的椭圆囊组织中,缺氧处理并未改变毛细胞谷氨酸的合成,但会显著增加毛细胞谷氨酸的释放。
英文摘要:
      Objective: To investigate the effects of hypoxia on the synthesis and release of glutamate acid in utricle hair cells in guinea pigs. Methods: Bilateral utricles of 4-week-old guinea pigs were isolated and treated in a constant oxygen (21% O2) and hypoxia (1% O2) condition for 30 minutes, respectively, in vitro. Western blotting and enzyme activity analysis were used to detect the activity and expression of glutaminase in hair cells and evaluate the change of synthesis of glutamate acid. The distribution of glutamate acid in the utricle were detected by ELISA and immunofluorescence. Results: After exposed to hypoxia, the expression and activity of glutaminase, which is the key enzyme for synthesis of glutamate acid, did not change significantly. However, compared to the control group, ELISA results showed a 5.6% reduction of glutamate levels in hypoxia group (P<0.05), the results of immunofluorescence indicated that glutamate acid was more widely distributed in the basal area than area that close to kinocilium in cytoplasm of utricle hair cells in hypoxia group. Conclusions: Hypoxia treatment did not change the glutamate synthesis of hair cells, but increased the release of glutamate acid significantly in utricle hair cells in vitro.
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