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| Jagged1蛋白在膝关节骨性关节炎软骨组织中的表达 |
| Expression of Jagged1 protein in cartilage tissue of knee osteoarthritis |
| 投稿时间：2020-02-11 修订日期：2020-03-23 |
| DOI： |
| 中文关键词: 膝关节骨性关节炎 Jagged1蛋白 Notch信号转导通路 关节软骨 |
| 英文关键词: knee osteoarthritis Jagged1 Notch signaling pathway articular cartilage |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 中文摘要: |
| 目的: 研究Jagged1蛋白在膝关节骨性关节炎关节软骨中表达的特点，探讨Jagged1蛋白的表达对关节软骨病理改变的影响。方法: 选取宁夏医科大学总医院骨科2018年11月1日至2019年10月31日34例因膝关节骨性关节炎行全膝关节置换的患者，取术中去除的股骨髁软骨组织，磨损较重的一侧为负重区（实验组），磨损较轻的一侧为非负重区（对照组）。通过番红、HE染色观察软骨组织形态学变化，通过免疫组织化学检测Jagged1蛋白在不同软骨组织中的表达强度，对比分析Jagged1蛋白表达的差异对关节软骨病变的影响。结果：外观照可见实验组软骨面色泽暗淡，表面粗糙，有大量软骨缺失，对照组软骨面较平整，呈白色，未见软骨明显缺失；HE染色可见实验组软骨面不整，软骨细胞排列紊乱，呈簇状分布，对照组软骨面平整，软骨细胞数量多，胞质丰富；番红染色见实验组软骨组织残留较少，软骨层结构模糊不清，软骨细胞较少，对照组软骨层次结构清晰，软骨细胞及软骨下骨细胞排列整齐；免疫组化可见Jagged1蛋白实验组软骨中有较多阳性表达，在对照组中表达较少，差异有统计学意义（P＜0.05）。结论: 在膝关节骨性关节炎的发生发展过程中，Jagged1蛋白参与了关节软骨的病变过程，且浓度随着软骨磨损程度的增加而升高，与膝骨性关节炎的严重程度密切相关。 |
| 英文摘要: |
| Objective: To study the expression characteristics of Jagged1 protein in articular cartilage of knee osteoarthritis, and to explore the effect of Jagged1 protein expression on the pathological changes of articular cartilage. Methods: Thirty-four patients who underwent total knee arthroplasty due to knee osteoarthritis were selected from the Department of Orthopaedics, General Hospital of Ningxia Medical University from November 1, 2018 to October 31, 2019. Take the?femoral condyle cartilage tissue removed during the operation, and the side with the heavier wear?was the weight-bearing area (experimental group), and the lighter side was the non-weight-bearing area (control group). The morphological changes of cartilage tissue were observed by saffron and HE staining. The expression intensity of Jagged1 protein in different cartilage tissues was detected by immunohistochemistry, and the effect of the difference in Jagged1 protein expression on articular cartilage lesions was compared and analyzed. Results: The appearance of the cartilage in the experimental group was dull and rough, and there were a large number of cartilage defects. The cartilage surface in the control group was flat and white with no obvious cartilage loss. The HE staining showed that the cartilage surface was irregular and the chondrocytes were arranged disorderly. The cartilage surface in the control group was flat, the number of chondrocytes was abundant, and the cytoplasm was rich. Saffron staining showed that there was less residual cartilage tissue, the structure of the cartilage layer was blurred, and there were fewer cartilage cells in the experimental group. The cartilage hierarchy in the control group was clear and the chondrocytes and subchondral osteocytes are neatly arranged. Immunohistochemistry showed that the Jagged1 protein in the experimental group had more positive expression in cartilage and less expression in the control group.Difference between the two groups has statistical significance.(P＜0.05) Conclusion: During the occurrence and development of knee osteoarthritis, Jagged1 protein is involved in the pathological process of articular cartilage, and its concentration increases with the increase of cartilage wear, which is closely related to the severity of knee osteoarthritis. |
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