文章摘要
臭椿生物碱canthin-6-one镇静催眠作用研究*
The effects of canthin-6-one extracted from Ailanthus altissima on sedation and hypnosis in mice
投稿时间:2019-08-21  修订日期:2019-08-25
DOI:
中文关键词: 铁屎米酮生物碱  镇静  催眠  神经递质
英文关键词: Ailanthus altissima  Sedation  Hypnosis  Neurotransmitters
基金项目:陕西省自然科学基础研究计划(No.2017JM8007);西安市2019年“科技+”行动计划-社会发展示范工程项目(No.J201902026)。
作者单位E-mail
权伟   
高凯   
陆云阳   
张伟   
汤海峰 空军军医大学 tanghaifeng71@163.com 
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中文摘要:
      目的 研究臭椿生物碱canthin-6-one的镇静催眠作用。方法 选昆明小鼠随机分为空白组、地西泮阳性药物组、canthin-6-one低、中、高给药组(5、10、20 mg·kg-1),采用阈上剂量和阈下剂量戊巴比妥钠诱导小鼠睡眠,记录入睡小鼠比例、睡眠潜伏期和睡眠持续时间。此外,酶联免疫法测定小鼠脑组织中5-HT、NE、DA和GABA含量。结果10、20 mg·kg-1 canthin-6-one可使小鼠的站立次数和自主活动次数均明显减少(P<0.05);可使阈上剂量戊巴比妥钠诱导的小鼠的睡眠潜伏期缩短(P<0.05),阈下剂量戊巴比妥钠诱导的小鼠入睡率提高(P<0.05)。此外,10、20 mg·kg-1 canthin-6-one也可降低小鼠脑组织中5-HT、NE含量、增加GABA含量(P<0.05)。结论 canthin-6-one有明显的协同戊巴比妥钠改善睡眠作用,其作用与相关神经递质(5-HT、NE、GABA)具有密切关系。
英文摘要:
      Objective To discuss the effects of canthin-6-one extracted from Ailanthus altissima on sedation and hypnosis in mice. Methods Kunming mice were randomly divided into a blank group, a diazepam-positive drug group, and a low, medium and high dose groups of canthin-6-one (5, 10, 20 mg·kg-1). Then, the mice were induced to sleep using pentobarbital sodium with suprathreshold and subthreshold dose by intraperitoneal injection. The proportion of sleeping, sleep latency and sleep duration of mice were recorded. The contents of 5-HT, NE, DA and GABA of brain tissue were measured by enzyme linked immunosorbent assay according to the manufacturer's instruction. Results The duration of spontaneous activity and standing was significantly decreased (P<0.05) by injected with 10, 20 mg·kg-1 of canthin-6-one. The proportion of sleeping mice with the subthreshold dose of pentobarbital sodium was significantly increased by 10, 20 mg·kg-1 canthin-6-one (P<0.05), the sleep latency and the sleep time with the suprathreshold dose of pentobarbital sodium were also changed (P<0.05). Moreover, the content of 5-HT, NE in brain tissues were markedly reduced by 10, 20 mg·kg-1 canthin-6-one (P<0.05); However, the content of GABA was markedly increased. (P<0.05) Conclusion Canthin-6-one had obvious synergistic effects on improving sleeping quality combined with pentobarbital sodium. The mechanism is correlated to neurotransmitter (5-HT, NE, GABA) release in the brain.
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