文章摘要
天麻素对镉诱导的星形胶质细胞损伤的保护效应
Protective effect of gastrodin on cadmium-induced injury of astrocytes of mice
投稿时间:2019-07-17  修订日期:2019-08-04
DOI:
中文关键词:   星形胶质细胞  GDNF  Nrf2  HO-1  SOD-1
英文关键词: Cadmium  Gastrodin  GDNF  Nrf2  HO-1  SOD-1
基金项目:国家自然科学(81630032;81401109);横向课题(2015BAI13B01);国家科技支撑计划(2016YFC1307100)
作者单位E-mail
周翠红 空军军医大学西京医院心身科 陕西 西安 zch4610@126.com 
薛姗姗 空军军医大学西京医院心身科 陕西 西安  
刘江正 空军军医大学毒理教研室 陕西 西安  
王化宁 空军军医大学西京医院心身科 陕西 西安  
顾婷婷 空军军医大学西京医院麻醉科 陕西 西安  
彭正午 空军军医大学西京医院心身科 陕西 西安 pengzhengwu1446@163.com 
 空军军医大学西京医院心身科 陕西 西安
空军军医大学毒理教研室 陕西 西安
空军军医大学西京医院麻醉科 陕西 西安 
 
摘要点击次数: 178
全文下载次数: 0
中文摘要:
      摘要 目的:探讨氯化镉(CdCl2)和天麻素(GAS)对小鼠星形胶质细胞活力及神经营养因子GDNF和 抗氧化基因Nrf2,HO-1,SOD-1表达的影响。方法:首先,给予体外培养的小鼠星形胶质细胞不同浓度的CdCl2(Con,2.5μM,5μM,10μM,20μM)处理24h或48h,随后检测细胞活力筛选出造成星形胶质细胞损伤的CdCl2浓度和时间。然后使用上述筛选的CdCl2作用浓度(5μM)构建星形胶质细胞损伤的同时再给予不同浓度的天麻素(0,20μg/mL,30μg/mL,40μg/mL, 50μg/mL)处理24h或48h, 随后检测细胞活力并提取细胞RNA检测其GDNF(胶质源性神经营养因子Glial cell-derived neurotrophic factor,GDNF)和Nrf2(Nuclear factor erythroid2-related factor2),HO-1(Heme oxygenase 1),SOD-1(superoxide dismutase 1)等抗氧化基因的mRNA表达的变化。结果: (1) 2.5μM CdCl2处理24h后星形胶质细胞活力已经有明显下降(P<0.05),5μM CdCl2处理24h后,星形胶质细胞活力显著下降(P<0.01);(2)CdCl2浓度越大,细胞损伤严重;(3)一定浓度的天麻素处理可以缓解CdCl2造成的星形胶质损伤;(4)CdCl2下调了星形胶质细胞的GDNF, Nrf2, HO-1和SOD-1的mRNA水平,天麻素可以抑制CdCl2对上诉基因的mRNA水平的调节作用,且浓度越高调节作用越强。结论:天麻素可能通过调节小鼠星形胶质细胞的GDNF, Nrf2, HO-1和SOD-1基因表达缓解CdCl2导致的细胞损伤。
英文摘要:
      Abstract: Objective To investigate the effect of cadmium and gastrodin on cell viability and the effect on the expression level of GDNF, Nrf2, HO-1 and SOD-1 of astrocytes from the hippocampus of mice. Methods: First, cultured hippocampal astrocytes from new-born (one day old) mice were divided into control and cadmium groups: control group was treated with medium(5%FBS+DMEM) and cadmium treatment groups were treated with 2.5, 5, 10 or 20 μM CdCl2 for 24 or 48 hours respectively. Then cell viability was measured by CCK-8 and the appropriate concentration and time point (5μM, 24h) of CdCl2 to induce cell injury were determined. After that, cultured astrocytes were divided into control, sham and GAS groups: control group was treated with medium(5%FBS+DMEM) , sham group was treated with 5μM CdCl2 for 24h, GAS groups were treated with 5μM CdCl2 and 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL gastrodin for 24h respectively at the same time. Then cell viability was measured by CCK-8 and total RNA was extracted from cells and the mRNA levels of GDNF, Nrf2, HO-1 and SOD-1 were measured by Real-time PCR. Results: (1) Compared with the control, CdCl2 significantly decreased the cell viability of astrocytes; (2) The effect of isoflurane revealed a dose effect---compared with 2.5μM group, cells treated with 5.0-20 μM of CdCl2 suffered more influence. (3) 5μM CdCl2 treated 24h significantly down-regulated the mRNA levels of GDNF, Nrf2, HO-1 and SOD-1 mRNA level in the astrocytes; (4) Gastrodin administration restored the cell viability and expression of GDNF, Nrf2, HO-1 and SOD-1 in the astrocytes post-CdCl2 exposures. Conclusion: Gastrodin administration restored the decreased cell viability and reversed the disturbed expression of GDNF, Nrf2, HO-1 and SOD-1 in astrocytes exposed to CdCl2.
View Fulltext   查看/发表评论  下载PDF阅读器
关闭