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| 氟苯达唑对人急性髓系白血病HL-60细胞的增殖抑制作用研究 |
| The inhibitory effect of flubenazole on proliferation of human acute myeloid leukemia HL-60 cells |
| 投稿时间：2019-05-21 修订日期：2019-06-04 |
| DOI： |
| 中文关键词: 氟苯达唑 急性髓系白血病 周期 凋亡 |
| 英文关键词: Fluendazole Acute myeloid leukemia Cell Cycle Apoptosis |
| 基金项目:福建省自然科学基金项目 |
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| 中文摘要: |
| 摘要 目的：研究氟苯达唑对人急性髓系白血病HL-60细胞增殖的抑制作用，明确氟苯达唑对HL-60细胞周期，凋亡发生的作用机制。方法：噻唑蓝法（MTT）检测氟苯达唑对人急性髓系白血病HL-60细胞的生长抑制作用，流式细胞术检测氟苯达唑对HL-60细胞周期，DNA片段化的影响，免疫印迹法检测Caspase,Raf,Bcl-2家族蛋白表达。结果：氟苯达唑抑制人急性髓系白血病HL-60细胞生长， HL-60细胞G2/M期增加， DNA片段化上升，促使Cleaved PARP, Cleaved-caspase 3, Cleaved-caspase 9蛋白表达量增加；Bag-1蛋白表达量降低，Bcl-2蛋白表达量降低增加；b-raf，c-raf磷酸化蛋白表达水平逐渐降低。结论：氟苯达唑通过诱导HL-60细胞阻滞于G2/M期，增加DNA片段化水平，激活Caspase, Raf, Bcl-2家族介导的凋亡相关通路抑制人急性髓系白血病HL-60细胞增殖，诱导人急性髓系白血病HL-60细胞发生凋亡而发挥抗肿瘤作用。 |
| 英文摘要: |
| ABSTRACT Objective:To investigate the inhibitory effect of flubendazole on the proliferation of human acute myeloid leukemia HL-60 cells, and clarifying the mechanism of flubendazole on cell cycle and apoptosis of HL-60 cells. Methods: Methyl thiazolyl tetrazolium (MTT) assay was used to evaluatethe growth inhibition effects of flubendazole on human acute myeloid leukemia HL-60 cells. Flow cytometry was used to detect the effect of flubendazole on HL-60 cell cycle and DNA fragmentation. Western blotting was used to detect the expression of Caspase, Raf, and Bcl-2 family proteins.Results: Flubendazole inhibited the growth of human acute myeloid leukemia HL-60 cells. The G2/M phase,the DNA fragmentation and the expression of Cleaved PARP, Cleaved-caspase 3, and Cleaved-caspase 9 of HL-60 cells increased.The expression of Bag-1 protein was decreased and the Bcl-2 was increased .the b-raf and the c-raf phosphorylation protein levels was inhibited by Flubendazole.Conclusions: The G2/M phase of human acute myeloid leukemia HL-60 cellswas arrested by Flubendazole, and increasing DNA fragmentation level and activating Caspase, Raf, and Bcl-2 family apoptosis-related pathways which Inducing apoptosis in human acute myeloid leukemia HL-60 cells and taking anti-tumor effects. |
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