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| 中频交变微电流联合紫杉醇抗A549细胞作用的研究 |
| Effect of Alternating Micro-current at Intermediate Frequency(ACIF) Combined with Paclitaxel on A549 Cells |
| 投稿时间：2019-05-20 修订日期：2019-05-20 |
| DOI： |
| 中文关键词: 中频交变微电流；紫杉醇注射液 A549细胞；细胞周期；凋亡； |
| 英文关键词: Alternating Micro-curren at Intermediate Frequency(ACIF) Paclitaxel injection A549 cells Cell cycle Apoptosis |
| 基金项目:国家自然科学基金项目（面上项目） |
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| 中文摘要: |
| 目的 研究中频交变微电流联合紫杉醇注射液抗A549细胞的作用及机制。方法 对处于对数生长期的肺腺癌A549细胞施加电刺激、紫杉醇及电刺激联合紫杉醇三种不同处理,采用MTT法检测A549细胞存活率,并利用流式细胞仪测量分析各组细胞凋亡/死亡比例及细胞周期状态。结果 本实验通过不同参数的中频交变微电流刺激A549细胞,得到的最低细胞存活率(参数150 kHz、90 mA、30 min)为78.02±0.73 %(P<0.01)；联合紫杉醇注射液半抑制浓度(IC50)干预后,细胞存活率为32.87±0.94 %(P<0.01)；同时发现中频交变微电流联合紫杉醇注射液能促进A549细胞凋亡,阻滞细胞于S期、G2/M期。结论 中频交变微电流可抑制A549细胞增殖、促进凋亡,但对细胞周期影响不明显；与紫杉醇注射液联合应用时具有协同增强抗肿瘤作用。 |
| 英文摘要: |
| Objective To study the effect and mechanism of Alternating Micro-current at Intermediate Frequency(ACIF) in combination with paclitaxel injection against A549 cells in vitro. Method The A549 cells was exposed to ACIF, paclitaxel and the combination of both in logarithmic phase. The cell growth rate was measured by MTT assay. Cell Apoptosis/death rate and cell cycle status were analyzed by flow cytometry. Result A549 cells were exposed to different ACIF parameters. The lowest cell survival rate was 78.02±0.73 % (parameter 150 kHz、90 mA、30 min) (P<0.01). After the intervention of paclitaxel (IC<sub>50</sub>), the cell survival rate was 32.87± 0.94 %(P<0.01). It was found that the combination of ACIF and paclitaxel can promote apoptosis of A549 cells, and block cells in S and G2/M phases. Conclusion ACIF can promote apoptosis of A549 cells, but has no obvious effect on cell cycle. When combined with paclitaxel injection, it has synergistic enhancement of anti-tumor effect. |
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