文章摘要
葛根素联合小檗碱干预非酒精性脂肪性肝炎两种动物模型的病理学研究
Pathological Study of Puerarin Combined with Berberine on Two Animal Models of Non-alcoholic Steatohepatitis
投稿时间:2019-02-17  修订日期:2019-02-17
DOI:
中文关键词: 葛根素  小檗碱  非酒精性脂肪性肝炎  大鼠  小鼠  病理学
英文关键词: Puerarin  Berberine  Non-alcoholic Steatohepatitis  Rats  Mice  Pathology
基金项目:北京市科技计划脑科学专项(Z161100000216136);国家自然科学基金青年科学基金项目(81503580)
作者单位E-mail
续畅 首都医科大学附属北京世纪坛医院 664107538@qq.com 
马致洁 首都医科大学附属北京友谊医院  
黄凤 首都医科大学附属北京中医医院  
钟晓松 首都医科大学附属北京世纪坛医院 664107538@qq.com 
摘要点击次数: 67
全文下载次数: 0
中文摘要:
      目的:从病理学的角度探讨葛根素及小檗碱对非酒精性脂肪性肝炎(NASH)的干预作用。方法:制备高脂饮食诱导的SD大鼠NASH模型及蛋氨酸-胆碱缺乏饮食诱导的C57BL/6小鼠NASH模型,各给药组造模并同时灌胃给药,大鼠持续8周后取材,小鼠持续2周后取材。肝组织制成石蜡切片进行HE染色,制成冰冻切片进行油红O染色,经光镜观察摄片后,采用NAFLD活动度积分对各组进行NASH评估。结果:葛根素、小檗碱可减轻两种NASH动物模型肝脏的脂肪变性、抑制肝细胞空泡化的发生,减少肝小叶内炎性病灶的数量。结论:葛根素及小檗碱可显著抑制NASH相关病理变化的发生发展,对NASH具有一定的干预作用。
英文摘要:
      Objective: To investigate the effect of puerarin and berberine on nonalcoholic steatohepatitis (NASH) from the pathological point of view. Method: The NASH model of SD rats induced by high fat diet and the NASH model of C57BL/6 mice induced by methionine-choline deficiency diet were prepared. The rats were taken after 8 weeks and the mice were taken after 2 weeks. Paraffin sections of liver tissue were stained with HE and frozen sections were stained with oil red O. After light microscopy, NAFLD activity score was used to evaluate NASH in each group. Results: Puerarin and berberine could alleviate steatosis, inhibit vacuolation of hepatocytes and reduce the number of inflammatory lesions in hepatic lobules in two NASH animal models. Conclusion: Puerarin and berberine can significantly inhibit the occurrence and development of pathological changes related to NASH, and have a certain intervention effect on NASH.
View Fulltext   查看/发表评论  下载PDF阅读器
关闭