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| 使用国产经济材料建立猪模型的体外肺灌注系统 |
| Using porcine models to evaluate an ex vivo lung perfusion system based on national economical materials |
| 投稿时间：2019-02-02 修订日期：2019-02-03 |
| DOI： |
| 中文关键词: 肺移植 体外肺灌注 肺功能 血气分析 |
| 英文关键词: Lung transplantation Ex vivo lung perfusion Pulmonary function Blood gas analysis |
| 基金项目:陕西省自然科学基础研究计划 |
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| 中文摘要: |
| 目的：肺移植是终末期肺部疾病的有效治疗方法,但供体肺的成功获取和有效维持是一个重要问题。体外肺灌注技术(Ex vivo lung perfusion, EVLP)已显示出巨大的潜力,但昂贵的成本令人望而却步。本文采用国产经济材料建立猪模型的EVLP系统,以探索提高供肺移植的适用性的同时降低移植费用。方法：我们首先通过国产材料配置肺灌注液,并组装管道,建立EVLP系统。然后我们通过手术手段获得了3头家猪的肺脏,并灌注肺灌注液,低温保存6小时。最后,我们将肺脏连接到EVLP系统,通过血气分析和肺功能检查来评估肺脏随时间变化的状况。结果：离体并在低温保存6小时的猪肺脏,通过我们建立的相对经济的EVLP系统,可以在2小时内维持了良好的氧合功能和肺生理指标。结论：我们建立的EVLP系统可以有效地维护离体猪肺的生理功能,且降低了成本,从而为肺移植体外肺灌注技术的优化应用提供了研究基础。 |
| 英文摘要: |
| Objective：Lung transplantation is an effective treatment for end-stage lung disease, but the successful acquisition and effective maintenance of donor lungs is a major problem. Ex vivo lung perfusion (EVLP) techniques have revealed great potential but expensive costs are prohibitive. Here we used national economical materials-based porcine models to modify the EVLP system in order to improve transplantation applicability of donor lungs and reduces expenses. Methods：We first configured the lung perfusate and assemble chambers via reasonably priced materials to established the EVLP system. Then we surgically obtained the lungs of three domestic pigs, perfused the lung perfusate, and stored them for 6 hours at low temperature. Finally, the lungs were connected to the EVLP system, and blood gas analysis and lung functional examination were used to assess the physiological function of the lungs over time. Results：By using this relatively low-priced EVLP system, we successfully obtained pig lungs and stored them for 6 hours at low temperature. Within 2 hours, the modified lung perfusion system maintained good oxygenation function and lung physiological indexes. Conclusions：The EVLP system we established can effectively acquire and store the physiological functions of the donor pig lungs. Besides such systems could reduce costs greatly. Together, what we done provided research basis for optimizing the ex vivo lung perfusion techniques for the application of lung transplantation. |
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