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| 兴奋性突触传入对皮层神经元形态发育的作用<sub><sup>*</sup></sub> |
| The effect of excitatory synaptic afferent signal on the morphological development of cortical neurons * |
| 投稿时间：2019-01-24 修订日期：2019-01-24 |
| DOI： |
| 中文关键词: 抑制性神经元 兴奋性神经元 AMPA受体 NMDA受体 形态发育 |
| 英文关键词: Inhibitory interneuron Excitatory neuron AMPA receptor NMDA receptor Morphological development |
| 基金项目:陕西省自然科学(2018KW-063),延安大学资政育人项目(YDZZYB17-092) |
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| 中文摘要: |
| 目的：抑制性神经元的正常发育对成熟大脑皮层的功能建立非常重要。为了检测离子型谷氨酸受体中的AMPA受体和NMDA受体对抑制性中间神经元以及兴奋性神经元的形态发育的具体调控作用,我们开展了本次实验。方法：我们采用原代培养皮层神经元、结合药物干预AMPA受体和/或NMDA受体的方法来阻断神经元的离子型谷氨酸受体,并利用GAD67-GFP鼠的绿色荧光来显示混合细胞群中抑制性神经元、CaMKII免疫荧光染色显示兴奋性神经元,随后在荧光显微镜下观察每组神经元形态的变化。结果：首先,当阻断AMPA和/或NMDA受体时,光镜下看到神经元网络的密度降低。其次,进一步分别观察两种神经元的变化。对于GFP阳性的抑制性神经元,当阻断AMPA和/或NMDA受体时,神经元突起分支数降低,突起长度缩短。对于CaMKII阳性的兴奋性神经元,当阻断AMPA和/或NMDA受体时,神经元的形态也出现类似变化,分支数降低,突起长度缩短。结论：在神经元发育的过程中,离子型谷氨酸受体介导的兴奋性突触传入,对抑制性神经元和兴奋性神经元的形态发育都非常重要,最终对神经环路的形成发挥重要的调控作用。 |
| 英文摘要: |
| Objective: The normal development of inhibitory neurons is important for the functional establishment of mature cerebral cortex. In order to detect the detailed regulatory effects of AMPA receptor and NMDA receptor within ionotropic glutamate receptors on the morphological development of inhibitory interneurons and excitatory neurons, we conducted this experiment. Methods: We applied primary cultured cortical neurons, combined with drug intervention of AMPA receptors and/or NMDA receptors, to block neuronal ionotropic glutamate receptors. Neurons of each group were observed under fluorescence microscope and the inhibitory neurons were displayed by green fluorescence in GAD67-GFP transgenic mice while the excitatory neurons were showed by CaMKII immunofluorescence staining. Results: First, when the AMPA and/or NMDA receptors were blocked, the density of the neural network decreased when observed under light microscope. Secondly, the changes of two different kinds of neurons were further observed. For GFP-positive inhibitory interneurons, when the AMPA and/or NMDA receptors were blocked, the number of neuronal branches decreased and the length of processes was shortened. For CaMKII-positive excitatory neurons, when the AMPA and/or NMDA receptors were blocked, the neurons showed similar changes in morphology, with reduced number of branches and shortened length of processes. Conclusion: During neuronal development, the excitatory synaptic afferent signal mediated by ionic glutamate receptor is very important for the morphological development of inhibitory interneurons and excitatory neurons, and finally plays an important regulatory role in the formation of neural circuits. |
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