文章摘要
生物磁谱分析技术在OA患者关节液磨屑颗粒评估中的初步应用*
A Preliminary Use of Bio-ferrography in the Study of Wear Debris Evaluation in Arthritic Joint Fluid*
投稿时间:2019-01-10  修订日期:2019-01-15
DOI:
中文关键词: 骨关节炎  生物磁谱分析  磨屑颗粒  早期诊断
英文关键词: Osteoarthritis  Bio-ferrography  Wear debris  Early diagnosis
基金项目:国家自然科学基金项目(青年基金项目);唐都医院科技创新发展基金
作者单位E-mail
牛舜 空军军医大学唐都医院骨科 西安 中国 21379067@qq.com 
龙华 空军军医大学唐都医院骨科 西安 中国  
张艳 空军军医大学唐都医院肿瘤科 西安 中国  
李永伟 空军军医大学唐都医院骨科 西安 中国  
赵振 空军军医大学唐都医院骨科 西安 中国  
马保安 空军军医大学唐都医院骨科 西安 中国 baoanmatd@qq.com 
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中文摘要:
      目的:随着人群的老龄化,骨关节炎(osteoarthritis,OA)已成为老年人最常见的疾病之一,严重影响老年人生活质量。而OA传统的诊断方法敏感性差,往往在确诊时,疾病已经发展到了晚期。本研究拟运用生物磁谱分析技术(bio-ferrography)来初步分析研究OA患者关节液中磨屑颗粒的参数,进而为OA的早期诊断提供依据。方法:选取符合纳入标准的2017年9月至2017年12月我科住院收治的OA患者。采集患者关节液后,运用bio-ferrography技术分离、收集关节液中的软骨磨屑颗粒和骨性磨屑颗粒,进一步通过扫描电镜(scanning electron microscope,SEM)观测磨屑颗粒的外形、数量和大小等参数。结果:随着患者OA等级的进展,软骨颗粒和骨性颗粒的数量均在增加,磨屑颗粒外形变得越来越锐利和不规则。在Kellgren-Lawrence(K-L)1级OA患者的关节液中,无骨性颗粒的存在,在K-L 1~3级OA患者的关节液中,软骨颗粒数量显著多于骨性颗粒。结论:我们初步探讨了通过bio-ferrography技术观测OA患者关节液中的磨屑颗粒,并评估了不同K-L分级OA患者关节液中磨屑颗粒的统计学特点,为今后建立以bio-ferrography技术为基础的OA早期诊断标准奠定了基础。
英文摘要:
      Objective: Osteoarthritis has been recognized as one of the most common diseases among old people, which has greatly reduced the quality of life. However, traditional OA diagnostic method lacks sensitivity that, most of the time, OA has developed to late stage until it was diagnosed. In this study, we aim to use bio-ferrography technique to analyze the wear particles in the synovial fluid of OA patients, which may provide references for early diagnosis of OA. Methods: OA hospitalized patients from 2019.09 to 2017.12 were selected according to our inclusion and exclusion criteria. The cartilage wear particles and bone wear particles in the synovial fluid were collected using bio-ferrography technique, and then, several parameters of these wear particles, such as shape, number and size, were observed by SEM. Results: The number of cartilage wear particles and bone wear particles increased with the OA classification, and the shape of these wear particles became more and more sharper and irregular. There were no bone wear particles in the synovial fluid of K-L 1 OA patients. Cartilage wear particles were more than bone wear particles in the synovial fluid of K-L 1~3 OA patients. Conclusion: In this study, a novel diagnostic method of bio-ferrography was used to briefly evaluate the wear particles in the synovial fluid of OA patients. Subsequently, the statistical characteristics of these wear particles from different OA classification patients were analyzed. This research established foundation to establish the OA early diagnostic criteria.
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