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| BRD4在喉鳞状细胞癌病灶转移过程中的作用及机制研究* |
| Role and mechanism of BRD4 in the process of focal squamous cell carcinoma metastasis |
| 投稿时间：2018-12-18 修订日期：2018-12-18 |
| DOI： |
| 中文关键词: BRD4 喉鳞状细胞癌 转移 侵袭 上皮间质转化 |
| 英文关键词: BRD4 Laryngeal Squamous Cell Carcinoma Metastasis Invasion EMT |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨BRD4在喉鳞状细胞癌病灶转移过程中的作用及其机制。方法：收集本院2016年4月-2018年4月收治的87例喉鳞状细胞癌患者手术标本。qRT-PCR、Western blot和免疫组化染色检测患者肿瘤组织(Tumor Tissue)、癌旁组织(Adjacent Tissue)和正常组织(Normal Tissue)中BRD4表达；BRD4 拮抗剂 GSK525762A(500 nmoL/L)处理喉鳞状细胞癌Hep2细胞,24、48和72 h后CCK-8检测细胞增殖；Trans-well细胞迁移实验和侵袭实验分别检测GSK525762A给药前后Hep2细胞迁移和侵袭能力；qRT-PCR 和Western blot检测GSK525762A给药前后Hep2细胞BRD4、E-Cadherin、N-Cadherin 和Twist蛋白表达。结果：喉鳞状细胞癌肿瘤组织中 BRD4表达高于癌旁组织和正常卵巢组织(P<0.05)；GSK525762A给药处理后Hep2细胞增殖能力、迁移和侵袭能力及BRD4和上皮间质转化相关蛋白N-Cadherin 和Twist表达均明显低于阴性对照组(P<0.05),而E-Cadherin表达的表达明显升高(P<0.05)。结论：BRD4可能通过抑制E-Cadherin的表达,增加N-Cadherin 和Twist的表达,进而激活上皮间质转化,促进喉鳞状细胞癌转移。 |
| 英文摘要: |
| Objective: To investigate the role and mechanism of BRD4 in the process of focal squamous cell carcinoma metastasis. Methods: Eighty-six surgical specimens of patients with laryngeal squamous cell carcinoma admitted from April 2016 to April 2018 were enrolled. qRT-PCR, Western blot and immunohistochemical staining were used to detect BRD4 expression in tumor tissues, paracancerous tissues and normal tissues; BRD4 antagonist GSK525762A (500 nmoL/L) was used to treat laryngeal squamous cell carcinoma Hep2 cells, 24, 48 and 72 After C, CCK-8 was used to detect cell proliferation; Trans-well cell migration assay and invasion assay were used to detect the migration and invasion of Hep2 cells before and after GSK525762A administration; qRT-PCR and Western blot were used to detect E-Cadherin、N-Cadherin and Twist |
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