文章摘要
BRD4在喉鳞状细胞癌病灶转移过程中的作用及机制研究*
Role and mechanism of BRD4 in the process of focal squamous cell carcinoma metastasis
投稿时间:2018-12-18  修订日期:2018-12-18
DOI:
中文关键词: BRD4  喉鳞状细胞癌  转移  侵袭  上皮间质转化
英文关键词: BRD4  Laryngeal Squamous Cell Carcinoma  Metastasis  Invasion  EMT
基金项目:
作者单位E-mail
吴小秦 海南省中医院 zxt3000@yeah.net 
谭业农   
唐旭霞 王翔 符国庆 李淑芳 海南省中医院 耳鼻喉科 海口 海南 中国  
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中文摘要:
      目的:探讨BRD4在喉鳞状细胞癌病灶转移过程中的作用及其机制。方法:收集本院2016年4月-2018年4月收治的87例喉鳞状细胞癌患者手术标本。qRT-PCR、Western blot和免疫组化染色检测患者肿瘤组织(Tumor Tissue)、癌旁组织(Adjacent Tissue)和正常组织(Normal Tissue)中BRD4表达;BRD4 拮抗剂 GSK525762A(500 nmoL/L)处理喉鳞状细胞癌Hep2细胞,24、48和72 h后CCK-8检测细胞增殖;Trans-well细胞迁移实验和侵袭实验分别检测GSK525762A给药前后Hep2细胞迁移和侵袭能力;qRT-PCR 和Western blot检测GSK525762A给药前后Hep2细胞BRD4、E-Cadherin、N-Cadherin 和Twist蛋白表达。结果:喉鳞状细胞癌肿瘤组织中 BRD4表达高于癌旁组织和正常卵巢组织(P<0.05);GSK525762A给药处理后Hep2细胞增殖能力、迁移和侵袭能力及BRD4和上皮间质转化相关蛋白N-Cadherin 和Twist表达均明显低于阴性对照组(P<0.05),而E-Cadherin表达的表达明显升高(P<0.05)。结论:BRD4可能通过抑制E-Cadherin的表达,增加N-Cadherin 和Twist的表达,进而激活上皮间质转化,促进喉鳞状细胞癌转移。
英文摘要:
      Objective: To investigate the role and mechanism of BRD4 in the process of focal squamous cell carcinoma metastasis. Methods: Eighty-six surgical specimens of patients with laryngeal squamous cell carcinoma admitted from April 2016 to April 2018 were enrolled. qRT-PCR, Western blot and immunohistochemical staining were used to detect BRD4 expression in tumor tissues, paracancerous tissues and normal tissues; BRD4 antagonist GSK525762A (500 nmoL/L) was used to treat laryngeal squamous cell carcinoma Hep2 cells, 24, 48 and 72 After C, CCK-8 was used to detect cell proliferation; Trans-well cell migration assay and invasion assay were used to detect the migration and invasion of Hep2 cells before and after GSK525762A administration; qRT-PCR and Western blot were used to detect E-Cadherin、N-Cadherin and Twist
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