| ABSTRACT Objective: To observe the imaging characteristics of a novel near-infrared fluorescence probe MHI85 in organ, and to search for specific organ imaging, so as to provide help for surgery. Methods: The optical properties of MHI85 was analyzed using an Ocean Spectrophotometer. The biodistribution and targeting was monitored in the CD-1 mouse 4 h post-injection of MHI85 using the Fluorescence-Assisted Resection and Exploration (FLARE?) real-time intraoperative imaging system. And intraoperative imaging of abdominal cavity, gallbladder and billary tract, resected organs was observed. Signal-to-background ratio (SBR) was calculated by comparing the signals of major organs against surrounding muscle. Results: The emission spectrum of MHI85 was at 700 nm, and imaging was stable. Real-time live-body imaging effectively reported the dynamic process of the biodistribution and clearance of MHI85 in vivo. And the signal of MHI85 targeting on gallbladder, gallbladder duct, left and right hepatic duct and hepatic duct of mouse was obviously strong at 4 h post-injection. There was no fluorescence signal in the heart, lung, liver, pancreas, spleen, kidney, duodenum and small intestine, while there was obvious fluorescence signal in the gallbladder. SBR of isolated organs was measured, indicating that the SBR of gallbladder was significantly higher than that of other organs. Conclusions: Near infrared fluorescence molecular probe MHI85 can be used as targets for imaging of gallbladder and biliary tract system because of its clear imaging and accurate localization. |
