文章摘要
900 MHz手机辐射对人视网膜色素上皮细胞增殖活性及转化生长因子β2表达的影响*
Effect of 900 MHz mobile phone radiation on proliferative activity and expression of transforming growth factor-β2 in human retinal pigment epithelium*
投稿时间:2018-08-28  修订日期:2018-09-12
DOI:
中文关键词: 手机  辐射  人视网膜色素上皮细胞  转化生长因子β2  眼部疾病
英文关键词: Mobile  Radiation  Human retinal pigment epithelium  Ttransforming growths factor-β2  Ocular disease
基金项目:上海市科委基金项目(No.17411950200 & 17411950206);上海市卫生局中医药科研基金(2014JP015A)
作者单位E-mail
徐艳春 上海交通大学医学院附属新华医院眼科 上海 200092 中国 1158382610@qq.com 
冯婕妤 上海交通大学医学院附属新华医院眼科 上海 200092 中国  
胡毅倩 上海交通大学医学院附属新华医院眼科 上海 200092 中国  
朱茂林 上海交通大学医学院附属新华医院眼科 上海 200092 中国  
朱煌 上海交通大学医学院附属新华医院眼科 上海 200092 中国 zhuhuang@xinhuamed.com.cn 
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中文摘要:
      目的:研究900 MHz手机辐射对人视网膜色素上皮细胞(RPE)增殖活性及转化生长因子β2(TGF-β2)表达的影响。 方法:RPE细胞体外培养和传代后分组。给予900 MHz手机电磁辐射者为辐射组,未给予辐射者为对照组。CCK8法检测RPE细胞的增殖活性。Real-Time PCR法检测TGF-β2 mRNA的表达。ELISA法检测RPE细胞培养上清液中TGF-β2的蛋白含量。结果:与对照组相比,辐射组RPE细胞的增殖活性降低,TGF-β2 mRNA表达增加,RPE细胞培养上清中TGF-β2含量上调,且差异具有统计学意义(P<0.05)。结论: 900 MHz的手机辐射在一定程度上影响了体外培养的RPE细胞的增殖活性及TGF-β2的表达与分泌,手机辐射有可能与受TGF-β2调控的眼部疾病相关。
英文摘要:
      Objective: To investigate the effect of 900 MHz mobile phone radiation on proliferative activity and expression of transforming growth factor-β2(TGF-β2) in human retinal pigment epithelium (RPE). Methods: RPE cells were cultured and passaged in vitro and divided into two groups. The radiation group was given exposure with a 900 MHz mobile phone in call and the control group were not given any exposure. Proliferation of RPE cells was detected by the cell-counting kit-8 (CCK8) assay. The expression of TGF-β2 mRNA and protein secretion were measured by Real-Time quantitative polymerase chain reaction (Real-Time PCR) and Enzyme-linked immunosorbent assay (ELISA), respectively. Results:The viability of RPE cells in radiation group was inhibited compared to the control group. The level of TGF-β2 mRNA and secretion was up-regulated under the exposure in contrast with the control group. The differences were statistically significant(P<0.05). Conclusions:900 MHz mobile phone radiation altered the proliferative activity and the expression of TGF-β2 in RPE cells in some experimental condition and might have an underlying association with some eye diseases related with TGF-β2.
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