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| 耐辐射奇球菌pprM基因增强大肠杆菌氧化抗性的研究 |
| Enhances of the antioxidant capacity in Escherichia coli by Deinococcus radiodurans pprM * |
| 投稿时间：2018-07-21 修订日期：2018-08-06 |
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| 英文关键词: |
| 基金项目:国家自然科学基金(81741143)；湖南省教育厅科研项目(17A186) |
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| 中文摘要: |
| 【】 目的 探讨耐辐射奇球菌pprM基因对大肠杆菌氧化抗性的影响。 方法 氯化钙法转化分别构建含pGEX-6p-1-pprM、pGEX-6p-1质粒的大肠杆菌DH5α。测定不同浓度H2O2对含pGEX-6p-1-pprM、pGEX-6p-1和野生型大肠杆菌DH5α活性的影响以及菌体内SOD/GSH/CAT水平的变化。 结果 与空质粒组和野生型组相比,含pprM的大肠杆菌在同浓度过氧化性情况下,其抑菌圈明显缩小,差异有统计学意义。与空质粒组和野生型组相比,含pprM的大肠杆菌体内CAT活力、SOD活性明显提高,但GSH量并没有明显提高。 结论 pprM基因能够提高大肠杆菌抗氧化能力,其机制可能与pprM基因增强细菌体内抗氧化酶的活性有关。 |
| 英文摘要: |
| Objective To study the effect of pprM gene on the antioxidant capacity of Escherichia coli and its mechanism. Method PGEX-6p-1-pprM plasmids were constructed and transferred into Escherichia coli. The bacterial inhibition zone test of pprM group, empty plasmid group and wild type Escherichia coli group were carried out with H2O2, and the diameter of bacteriostatic ring was measured. Three types of Escherichia coli groups were respectively treated with 0 umol. L (PBS solution), 5 umol. L and 10 umol L for 30 minutes. The bacterial protein was extracted by centrifugation in the ice bath. The quantity of GSH and activity of SOD and CAT were measured by corresponding kits, respectively. Result Compared with empty plasmid group and wild type group, pprM group showed a significant smaller bacteriostatic ring under the same concentration peroxide circumstance in the bacterial inhibition zone test (P<0.05).The pprM group also showed a significant increasement of SOD and CAT activity (P<0.05), but the increasement of GSH quantity did not reach the significance (P<0.05). Conclusion The above results suggest that Deinococcus Radiodurans pprM gene can enhance the antioxidant capacity of E. coli and the mechanism may due to its improvement of antioxidase in bacteria. |
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