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| 五加生化胶囊对药物流产出血模型大鼠的保护作用及机制研究 |
| Protective effect and Mechanism of Wujia biochemical capsule on Rats with Drug abortion and Hemorrhage |
| 投稿时间：2018-07-13 修订日期：2018-07-13 |
| DOI： |
| 中文关键词: 五加生化胶囊 药物流产出血 雌二醇 雌激素受体 米非司酮 |
| 英文关键词: Acanthopanax senticosus capsule Drug abortion bleeding Estradiol Estrogen Receptor Mifepristone |
| 基金项目:] 黑龙江省科技攻关计划项目(GC10C208)；哈尔滨市科技创新人才研究专项资金(2016RQYXJ012)；黑龙江中医药大学校科研(201516,201726)；黑龙江省博士后资助项目(LB-Z16200)*[通讯作者] 张宁,中药学博士,研究员,硕士生导师,主要从事中药药效物质基础及体内代谢研究,Tel：:( 0454 ) 6050350,E-mail: zhangning0454@163.com耿放,中药学博士,副教授,硕士生导师,从事天然药物活性成分研究,Tel：:( 0451 ) 82475863,E mail：gengfang1980@163.com ,耿放2*,刘国良1,王瑶1,李铁男1,王发善1,张宁1,3* |
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| 中文摘要: |
| 目的：观察五加生化胶囊对药物(米非司酮配伍米索前列醇)致流产出血大鼠模型的保护作用,并探讨其作用机制。方法：建立大鼠药物流产出血模型,分为正常组(K)、受孕对照组(KY)、模型组(M)和给药(G)组,观测大鼠子宫出血时间、出血量、子宫指数、子宫形态和血液流变学改变,采用放射免疫法、ELISA法、免疫组织化学法和IPP6.0图像分析系统对比观察了大鼠血清雌二醇(E2)、血浆层粘连蛋白(LM)和纤维连接蛋白(FN)含量变化,以及子宫组织中FN、LM和雌、孕激素受体(ER、PR)表达的变化。结果：与M组比较,G组药物流产出血大鼠子宫血量和出血时间明显减少,血清E2含量和子宫ERα和PR的表达升高,血浆和子宫组织LM和FN水平降低,血液流变性得以改善。结论：五加生化胶囊具有减少药物流产出血大鼠子宫出血量和出血时间的作用,其作用机制与调节血清E2、血浆LM及FN水平,以及调节子宫组织ERα、PR和FN的表达有着密切关联。 |
| 英文摘要: |
| Objective To observe the protective effect of Wujia Shenghua Capsule on rats with hemorrhage induced by mifepristone and misoprostol and to explore its mechanism. Method Establishment of a rat model of drug abortion and hemorrhage,Divided into normal group (K), pregnant control group (KY), model group (M) and administration group (G),Observe the Changes of uterine bleeding time, bleeding volume, uterine index, uterine morphology and hemorheology in rats,the changes of serum estradiol (E2), plasma laminin (LM) and fibronectin (FN) in rats were observed by radioimmunoassay, immunohistochemistry and IPP6.0 image analysis system,Changes of expression of FNLM, estrogen and progesterone receptor (ERP,PR) in uterus.Results Compared with M group, the uterine blood volume and bleeding time were significantly decreased, serum E2 content and the expression of ER α and PR in uterus were increased, the levels of LM and FN in plasma and uterine tissue were decreased, and the hemorheology was improved in group G.Conclusion Wujia Shenghua capsule can reduce the amount and time of uterine bleeding in rats with drug abortion hemorrhage, and its mechanism is closely related to regulating the levels of serum E2, plasma LM and FN, and regulating the expression of ER α -PR and FN in uterine tissue. |
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