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| 基于深圳男男同性性行为人群的HIV-1广谱中和抗体筛选分析* |
| Screening and analysis of HIV-1 broadly neutralizing antibodies based on Shenzhen MSM corhot * |
| 投稿时间：2017-02-20 修订日期：2017-02-23 |
| DOI： |
| 中文关键词: 人类免疫缺陷病毒 广谱中和抗体 男男同性性行为人群 |
| 英文关键词: Human Immunodeficiency Virus Broadly neutralizing antibodies Men who have sex with men |
| 基金项目:人社部2016年度回国留学人员科技活动择优资助项目(无编号)；深圳市科技创新委基础研究学科布局项目(JCYJ20160331173336891)、基础研究项目(JCYJ20150402102135510)。 |
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| 中文摘要: |
| 目的 筛选基于深圳本地男男同性性行为者(Men who have sex with men,MSM)人群队列中HIV-1流行毒株的广谱中和抗体(Broadly neutralizing antibodies,bnAbs),为下一步机制和应用研究打基础。 方法 建立小型MSM队列,按计划分别定期随访、留样,测序分析人群中HIV-1病毒流行亚型。选取将骨架质粒与系列标准HIV-1 env质粒12款,分别共转染293细胞制备单次感染能力假病毒。建立TZM-bl细胞实验测定并计算中和活性(ID50 Titers)技术平台,并用于选定病毒亚型样品的筛选。最后取所获具有一定广谱中和抗性的代表性血样,抗体竞争实验初步分析其结合位点。 结果 近年来深圳MSM 的HIV-1流行亚型分布中,CRF07_BC(43.4 %)和CRF55_01B(15.4 %)占比快速增长。选择来自该人群队列CRF07_BC感染者34人88份血样进行广谱中和抗性检测,筛选出具有一定广谱中和抗性的血样10份(ID50 Titer≧25),其中2例显示了较佳中和宽度,可作用于全部12种假病毒中的7种(58.3 %)。初步分析其结合机制均非靶向gp120。 结论 深圳地区MSM人群中HIV-1毒株进化和流行特性日益复杂,对其中增长迅速重组亚型的bnAbs相关研究亟待加强。通过本研究已成功建立小型MSM队列和TZM-bl检测分析技术并应用于实践。初步筛选结果提示部分具有中和活性的患者血清内存在bnAbs。 |
| 英文摘要: |
| Objective：To screen broadly neutralizing antibodies(bnAbs) of HIV-1 epidemic strains in Men who have sex with men (MSM) lived in Shenzhen, and lay a basis for next mechanism and application research. Methods： An small corhot of local MSM were established. Participants were follow up and retention samples regularly, HIV-1 subtype distribution from these sampled local MSM was also analyzed monitored by sequencing. In this study, we prepared 12 type of Standard HIV-1 Env-pseudotyped viruses, which were produced by co-transfecting 293T cells with env expression plasmid and HIV-1 backbone expression plasmid, for TZM-bl-based neutralization assay. Selected samples from local MSM corhot were detected and ID50 titers were calculated through sera neutralization curves. Individuals showed relatively more broad or potent neutralizing activity would be analyzed by additional neutralization competition assay. Results： HIV-1 subtype distribution from local MSM significantly changed in recently years, The prevalence of CRF07_BC（43.4 %）and CRF55_01B(15.4 %) increased rapidly. We detected 88 samples from 34 MSM with CRF07_BC infection, 10 samples from 6 individuals shown cross-clade neutralizing activity, 2 of them showed plasma neutralization breadth of 58.3 % against a multi-subtype panel, the primary analysis indicated that the plasma neutralization in these two individuals is not directed to gp120. Conclusions：Genetic and epidemical properties of HIV-1 have become increasingly complex among Shenzhen MSM. The importence of bnAbs related research on these rapidly increased HIV-1 inter-subtype recombinants should be highlighted. In this study, we established local MSM cohort, TZM-bl-based neutralization assay technology platform, and successfully applied to practice. Primary results indicated part of samples exist HIV-1 bnAbs. |
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