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| 淫羊藿苷对大鼠正畸牙齿压力区牙周组织RANKL和Wnt3a的影响 |
| The effect of icariin on expression of RANKL and Wnt3a during orthodontic tooth movement in the pressure zone of periodontal tissue |
| 投稿时间：2017-01-10 修订日期：2017-01-16 |
| DOI： |
| 中文关键词: RANKL Wnt3a 淫羊藿苷 牙周组织 |
| 英文关键词: RANKL Wnt3a Icariin Periodontal tissue |
| 基金项目:黑龙江省博士后科研启动金（LBH-Q11033） |
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| 中文摘要: |
| 摘要 目的：观察在不同浓度的淫羊藿苷作用下，大鼠正畸牙齿移动时压力区牙周组织中RANKL和Wnt3a的表达变化。方法：将24只健康雄性SD大鼠随机分为4组，根据淫羊藿苷灌胃的浓度分为生理盐水组(对照组)和1 mg/kg淫羊藿苷组；3 mg/kg淫羊藿苷组；5 mg/kg淫羊藿苷组(实验组)。使用50 g力近中移动左侧上颌第一磨牙。通过免疫组化方法检测压力区牙周组织中RANKL和Wnt3a蛋白的表达。用SPSS17.0软件进行统计学分析。结果：RANKL和Wnt3a在生理盐水组和淫羊藿苷组的压力区牙周组织中都有表达。RANKL的表达随淫羊藿苷浓度的增加而逐渐减少(P <0.05)。Wnt3a的表达随淫羊藿苷浓度的增加明显增加(P <0.05)；结论：淫羊藿苷能减少正畸时牙齿移动过程中压力区牙周组织中RANKL的表达，增加Wnt3a的表达。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of different concentration icariin on the changes of RANKL and Wnt3a expressions in the pressure zone of periodontal tissue during orthodontic tooth movement(OTM). Methods: Twenty four male SD rats were randomly divided into four groups according to the different concentrations of icariin: 1 mg/kg; 3 mg/kg; 5 mg/kg groups(experience group) and physiological saline group(control group). Using 50 g force to move the left maxillary first molar. Immunohistochemical staining technology was used to detect the expression of RANKL and Wnt3a in the pressure zone of periodontal tissue. SPSS17.0 software was used for statistical analysis. Results: RANKL and Wnt3a were expressed on both the physiological saline and icariin groups in the pressure zone of periodontal tissue. The expression of RANKL was decreased gradually with the increased concentration(P<0.05). The expression of Wnt3a was increased gradually with the increased concentration(P<0.05). Conclusions: Icariin can decreased the expressed of RANKL and increase the expressed of Wnt3a in periodontal tissue remodeling during OTM. |
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