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目的：探讨EGCG对蛛网膜下腔出血(SAH)脑损伤的影响，阐明其保护作用机制。方法：选取健康SD雄性大鼠80只，按照随机数字表法分为Sham组、SAH组、SAH+EGCG组、SAH+NS组，各20只。SAH造模成功后1d、3d，检测大鼠神经功能评分及脑组织含水量；造模成功3d后脑组织石蜡切片HE染色观察脑细胞形态，并采用hsCRP试剂盒检测血清hsCRP水平，及通过Western blotting检测脑组织BDNF、TrkB表达量。结论：1、SAH+EGCG组神经功能评分较Sham组明显降低，但较同期SAH组增高明显，差异具有统计学意义（p<0.05）；2、与Sham组比较，SAH+EGCG组脑组织含水量明显增加，但与同期SAH组比较，SAH+EGCG组脑组织含水量明显降低，差异显著，具有统计学意义（p<0.05）；3、与SAH组比较，SAH+EGCG组血清hsCRP水平明显降低，差异显著，具有统计学意义（p<0.05）；4、与SAH组比较，SAH+EGCG组脑组织石蜡切片HE染色神经细胞水肿明显减轻，且坏死神经元减少，细胞核固缩明显减轻；5、与SAH组比较，SAH+EGCG组脑组织BDNF、TrkB的表达量明显升高。结论：大鼠SAH后给予EGCG干预，能够明显减轻脑组织水肿、脑细胞死亡，降低血清hsCRP水平，并改善大鼠神经行为学评分，促进脑组织BDNF、TrkB的表达，表明EGCG能够有效的保护大鼠SAH后的脑损伤，促进神经功能修复，减轻预后神经功能障碍。

英文摘要:

Objective To investigate the effects of EGCG on brain injury induced by subarachnoid hemorrhage (SAH) and elucidate its protective mechanism. Methods 80 healthy SD male rats were randomly divided into Sham group (n=20), SAH group (n=20), SAH+EGCG group (n=20), SAH+NS group (n=20).We established SAH model animals. The we measured the neurological function score and brain water content after the success of the model 1D, 3D, and observed the brain cells morphology by HE staining of paraffin section brain tissue at 3D, and detected the levels of serum hsCRP by hsCRP kit at 3D, and detected the expression of BDNF and TrkB in brain tissue by Western blotting at 3D. Results 1.Compared with the Sham group, the neurological function score of the SAH+EGCG group was lower, however, the neurological function score of the SAH+EGCG group was higher than the SAH group at the same period, the difference was statistically significant (p<0.05); 2.Compared with the Sham group, the brain water content of the SAH+EGCG group was significantly increased, but compared with the SAH group, the brain water content of the SAH EGCG group was significantly lower, the difference was statistically significant (p<0.05). 3. Compared with the SAH group, the serum hsCRP level in the SAH+EGCG group was significantly lower, the difference was statistically significant (p<0.05). 4. Compared with the SAH group, the SAH+EGCG group of the brain tissue paraffin sections stained with HE staining showed that the edema of nerve cells was decreased, and the necrotic neurons were reduced, and the nuclear pyknosis was significantly reduced.5. Compared with the SAH group, the expression of BDNF and TrkB in brain tissue was significantly increased. Conclusion After SAH in rats, given EGCG intervention can significantly reduce the brain edema, brain cell death, and reduce serum hsCRP levels, and improve the neurobehavioral score, and promote the expression of BDNF and TrkB in brain tissue, showed that EGCG can effectively protect the brain injury induced by SAH, and promote nerve function recovery, and reduce the prognosis nerve dysfunction.

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