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| 钩藤散改善APP/PS1双转基因小鼠学习记忆功能的代谢组学研究 |
| The metabolomic study of learning and memory function of APP/PS1 double transgenic mice improved by Gouteng san |
| 投稿时间：2017-01-04 修订日期：2017-02-08 |
| DOI： |
| 中文关键词: 钩藤散 APP/PS1双转基因小鼠 Morris水迷宫 代谢组学 潜在靶点 |
| 英文关键词: Gouteng san APP/PS1 transgenic mice Morris water maze metabolomics potential target |
| 基金项目: |
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| 摘要点击次数: 516 |
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| 中文摘要: |
| 目的 采用非靶向的高通量尿液代谢组学技术对钩藤散改善APP/PS1双转基因小鼠的作用机制进行研究。方法 5月龄APP/PSI小鼠采用Morris水迷宫实验检测双转基因小鼠的空间学习能力,在确定出现空间记忆能力功能损伤地条件下采用基于非靶向的尿液代谢组学技术研究APP/PSI小鼠的代谢网络,聚焦关键通路,同时观察钩藤散在水迷宫和代谢水平上的治疗作用。结果 Morris水迷宫对比发现APP/PSI小鼠的空间记忆能力明显长于同窝野生小鼠,给予钩藤散后呈现一定程度的回调趋势,经非靶向的代谢轮廓分析和核心代谢通路聚焦后,成功发现正常小鼠(同窝野生小鼠)和APP/PSI双转基因小鼠代谢轮廓间差异最大的信号,经质谱解析和权威数据库检索后鉴定6个与学习记忆相关的潜在生物标记物,分别是牛磺酸(taurine)、叶酸(pteroylglutamic acid)、新蝶呤(neopterin)、磺乙谷酰胺(glutaurine)、戊邻酮二酸盐(2-oxoglutarate)、二氢新蝶呤(dihydroneopterin),他们主要涉及牛磺酸代谢及叶酸代谢等,经钩藤散治疗后能有效回调。结论 钩藤散对APP/PSI双转基因小鼠的学习记忆能力具有一定治疗作用,本次发现的6个生物标记物可能是APP/PSI双转基因小鼠发病的潜在靶点,给予钩藤散后这些标记物呈不同程度的回调趋势,提示钩藤散可能通过调节相关代谢酶及代谢通路达到治疗学习记忆功能损伤的目的,为钩藤散的相关药效学研究提供实验依据。 |
| 英文摘要: |
| Study on the mechanism of non targeted high-throughput urine metabolomics powder improve APP/PS1 double transgenic mice of Uncaria by the. Methods May old APP/PSI mice by Morris water maze test for transgenic mice spatial learning ability, the metabolic network based on non target urine metabolic technology to study APP/PSI mice in the condition of spatial memory impairment by focusing the key pathway, while observing the treatment effect of Gouteng san on water maze and metabolism the. The results of Morris water maze compared the spatial memory ability of APP/PSI mice was significantly longer than the littermate wild-type mice, given the callback trend of Gouteng san after a certain degree, the non targeted metabolic profiling and central metabolic pathways after focusing, successfully found in normal mice (littermate wild-type mice) signal differences between transgenic mice and metabolic profile APP/PSI is the largest, by mass spectrometry analysis and identification of 6 authoritative database retrieval after learning and memory related potential biomarkers, which are taurine (taurine), folic acid (pteroylglutamic acid), methotrexate (neopterin), methyl ethylene (glutaurine), glutamine 2-oxoglutarate (2-oxoglutarate), two h neopterin (dihydroneopterin), they were mainly related to taurine metabolism and folate metabolism, the treatment of Gouteng san effectively after the callback. Conclusion Goutengsan on learning and memory ability of APP/PSI transgenic mice has a certain therapeutic effect, 6 biomarkers of this discovery may be a potential target of APP/PSI double transgenic mice given the incidence of these markers was scattered after the Uncaria callback trend in different degree, suggesting that Gouteng san could regulate metabolism related enzymes and metabolic pathways to treat the damage of learning and memory to provide the experimental basis for the study of the effects of Gouteng san. |
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