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| miRNA-26a靶向调控GSK-3β参与Wnt/β-catenin信号通路调节IgA肾病肾纤维化 |
| MicroRNA-26a participation the wnt/β-catenin signaling pathway induced renal fibrosis by target GSK-3β in IgA nephropathy |
| 投稿时间：2016-10-28 修订日期：2016-10-28 |
| DOI： |
| 中文关键词: IgA肾病 miR-26a Wnt/β-catenin信号通路 |
| 英文关键词: IgA nephropathy MicroRNA-26a Wnt/β-catenin signaling pathway |
| 基金项目: |
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| 中文摘要: |
| 目的：通过检测IgA肾病不同程度肾间质纤维化患者肾组织miR-26a、β-catenin、GSK-3β、α-SMA的表达,探究miR-26a通过靶向调控GSK-3β参与Wnt/β-catenin信号通路所致肾间质纤维化。方法：根据肾间质纤维化程度将46例IgA患者分为3组,对照组为7例肾脏肿瘤远离肿瘤组织的正常肾组织。采用RT-qPCR方法检测各实验组及正常对照组共52例IgA肾病患者肾组织miRNA-26a的表达水平,分析miR-26a与IgA肾病肾纤维化的关系。采用免疫组化技术检测各组肾组织β-catenin、GSK-3β、α-SMA的表达,各组之间进行比较,并与miR-26a的变化进行相关性分析。结果：(1)IgA 肾病不同程度肾间质纤维化患者肾活检组织miR-26a表达较正常对照组降低,且随着肾间质病变程度加重,miR-26a表达显著降低,与对照组相比差异具有统计学意义(P＜0.05)；(2)在IgA肾病患者,随着肾间质病变程度加重,GSK-3β、β-catenin、α-SMA表达水平增加,与对照组相比差异具与有统计学意义(P＜0.05),肾组织miR-26a与肾间质纤维化程度呈负相关(r=-0.943,P＜0.05),肾间质及肾小管GSK-3β、β-catenin、α-SMA的表达强度与肾间质纤维化程度正相关(r =0.917,P＜0.05；r =0.943,P＜0.05；r =0.926,P＜0.05)。结论：miR-26a可通过靶向调控GSK-3β参与Wnt/β-catenin信号通路肾间质纤维化。 |
| 英文摘要: |
| Objective:Detecting the expression of microRNA-26a,GSK-3β, β-catenin,α-SMA in varying degrees renal fibrosis patients in IgA nephropathy,to exploration microRNA-26a participation the Wnt/β-catenin signaling pathway induced renal fibrosis by target GSK-3β in IgA nephropathy. Methods: According to the degree of tubulointerstitial fibrosis ,46 cases of patients with IgA nephropathy were divided into three groups. 7 cases normal renal tissues adjacent to cancer were selected as control group in the same periods. To detection the expression of mircoRNA-26a in renal biopsy in normal control and the experiment group by RT-qPCR. Immunohistochemistry staining method was employed to detect the expression of GSK-3β, β-catenin, α-SMA in normal and experiment group. Analysis the level of microRNA-26a,GSK-3β,β-catenin, α-SMA in patients with IgA nephropathy, further,analysis the correlation among the level of microRNA-26a,GSK-3β, β-catenin, α-SMA and renal tubular interstitial fibrosis. Results :(1) Compared with the normal group, the expression of mircoRNA-26a decrease in the patients with IgA nephropathy. With the aggravate of tubulointerstitial fibrosis, the expression of microRNA-26a significantly decrease(P<0.05). (2) Compared with the normal group, the expression of GSK-3β、β-catenin、α-SMA were increased(P<0.05),the expression of miR-26a in renal were negtively correlated with the development of renal interstitial（r=-0.943，P＜0.05）,the expression of GSK-3β、β-catenin、α-SMA in tubulointerstitium were positively with the development of renal fibrosis（r =0.917，P＜0.05；r =0.943，P＜0.05；r =0.926，P＜0.05），。Conclusion: MicroRNA-26a participation the wnt/β-catenin signaling pathway induced renal fibrosis by target GSK-3β in IgA nephropathy. |
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