文章摘要
阻断RAS对HSkMCs细胞株细胞凋亡及Mfn2表达的影响蔡俊玮 李雪锋 黄成虎 徐焱成
Cai jun-wei, Li xue-feng, Huang Cheng-hu, Xu yan-cheng.The effect of blocking Renin-angiotensin system on the expression of mfn2 mRNA and apoptosis in HSkMCs
投稿时间:2014-05-16  修订日期:2014-05-16
DOI:
中文关键词: 局部  肾素-血管紧张素系统(RAS)  胰岛素抵抗  线粒体融合蛋白2(mfn2)
英文关键词: local  renin angiotensin system(RAS)  insulin resistance  mitofusin 2(mfn2)  human
基金项目:湖北省教育厅科学技术研究计划重点项目(D20112105);湖北省十堰市科技局项目(2010st12);湖北医药学院2010博士启动项目(2010QDJ24);湖北医药学院附属太和医院2010博士启动项目(2010QD15)作者单位:442000 湖北医药学院附属太和医院内分泌科(蔡俊玮、李雪锋、黄成虎);武汉大学中南医院内分泌科(徐焱成)通信作者:李雪锋,Email:ymclixf0629@163.comSS
作者单位邮编
蔡俊玮 十堰市太和医院(湖北医药学院附属医院) 442000
李雪锋* 十堰市太和医院(湖北医药学院附属医院) 442000
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中文摘要:
      【】 目的 观察氯沙坦对高糖培养人骨骼肌细胞株(HSkMCs)mfn2表达及细胞凋亡的影响。 方法 1.不同葡萄糖浓度培养基(5.55mmol/l葡萄糖组,11.1mmol/l22葡萄糖组,22.2mmol/l葡萄糖组)分别培养HSkMCs细胞株48小时,检测各组的AT1R、mfn2基因表达,流式细胞术检测细胞凋亡。 2.从上述实验选择对线粒体融合蛋白mfn2 影响最大的葡萄糖浓度(此组葡萄糖浓度为22.2mmol/L)作为下列实验的条件,加入血管紧张素受体Ⅱ拮抗剂(ARB)氯沙坦(Losartan),处理人骨骼肌细胞(HSkMCs)48h,以未加氯沙坦为对照组,观察其对线粒体融合蛋白2(mfn2)表达的影响,并行流式细胞术检测细胞凋亡。 结果 氯沙坦干预组HSkMCs细胞株mfn2表达上调,细胞凋亡减少。 结论 阻断RAS能上调HSkMCs细胞株mfn2表达,并减少细胞凋亡。
英文摘要:
      【】 Object To observe the effect of losartan on the expression of mfn2 mRNA and apoptosis in Human skeletal muscle cells(HSkMCs) cultured by high glucose medium. Methods SkMCs were cultivated in vitro and treated with glucose of different concentration(5.55mmol/l group, 11.1mmol/l group, 22.2mmol/l group) for 48 hours. Then the expression of AT1R and mfn2 was detected by PCR, apoptosis was detected by flow cytometry. 2. Select 22.mmol/l group which has the most influence on the expression of mfn2 from the above experiments as the following experimental conditions. Added losartan ( ARB ) to treat HSkMCs for 48h, in compared with the group without losartan. To observe its effect on the expression of mfn2 and apoptosis via flow cytometry . Results he expression of mfn2 in HSkMCs cultivated added losatan group was up – regulated, while apoptosis was reduced. Conclusion Blocking RAS can upregulate the expression of mfn2 and reduce cell apoptosis in HSkMCs,
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