| .LncRNA TUG1、miR-29a-3p表达与特发性肺纤维化患者病变程度、
肺功能及PI3K/Akt/mTOR信号通路的关系研究[J].,2024,(24):4766-4768 |
| LncRNA TUG1、miR-29a-3p表达与特发性肺纤维化患者病变程度、
肺功能及PI3K/Akt/mTOR信号通路的关系研究 |
| Study on the Relationship between the Expression of Serum LncRNA TUG1 and miR-29a-3p and the Degree of Lesion, Lung Function and PI3K/Akt/mTOR Signaling Pathwayin Patients with Idiopathic Pulmonary Fibrosis |
| 投稿时间：2024-08-09 修订日期：2024-08-26 |
| DOI：10.13241/j.cnki.pmb.2024.24.041 |
| 中文关键词: LncRNA TUG1 miR-29a-3p 特发性肺纤维化 病变程度 肺功能 PI3K/Akt/mTOR信号通路 |
| 英文关键词: LncRNA TUG1 miR-29a-3p Idiopathic pulmonary fibrosis Degree of lesion Lung function PI3K/Akt/mTOR signaling pathway |
| 基金项目:广西壮族自治区卫生和计划生育委员会科研课题（Z20170295） |
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| 中文摘要: |
| 摘要 目的：探讨血清长链非编码核糖核酸牛磺酸上调基因1（LncRNA TUG1）、微小核糖核酸-29a-3p（miR-29a-3p）表达与特发性肺纤维化（IPF）患者病变程度、肺功能及磷脂酰肌醇3 激酶（PI3K）/蛋白激酶B（Akt）/雷帕霉素靶蛋白（mTOR）信号通路的关系。方法：选取2021年1月至2023年12月期间我院收治的IPF 患者52例（研究组），另选取同期于我院体检健康的志愿者50例（对照组）。比较两组血清LncRNA TUG1、miR-29a-3p水平、肺功能参数[肺总量（TLC）、第1 秒用力呼气容积占预计值百分比（FEV1%pred）、用力肺活量（FVC）、一氧化碳弥散量占预计值百分比（DLCO%pred）]、高分辨率电子计算机断层扫描（HRCT）评分及PI3K、Akt、mTOR mRNA表达。采用Pearson法分析相关性。结果：研究组血清LncRNA TUG1、PI3K、Akt、mTOR mRNA表达水平高于对照组，miR-29a-3p表达水平低于对照组（P＜0.05）。研究组TLC、FEV1%pred、FVC、DLCO%pred低于对照组，HRCT评分高于对照组（P＜0.05）。血清LncRNA TUG1表达与HRCT评分、PI3K、Akt、mTOR mRNA表达呈正相关，与TLC、FEV1%pred、FVC、DLCO%pred呈负相关（P＜0.05）；miR-29a-3p与HRCT评分、PI3K、Akt、mTOR mRNA表达呈负相关，与TLC、FEV1%pred、FVC、DLCO%pred呈正相关（P＜0.05）。结论：IPF患者血清LncRNA TUG1呈高表达，miR-29a-3p呈低表达，二者与IPF患者肺组织病变程度、肺功能受损程度及PI3K/Akt/mTOR信号通路异常激活密切相关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between the expression of serum long non-coding RNA taurine up-regulated gene 1 (LncRNA TUG1) and microRNA-29a-3p (miR-29a-3p) and the degree of lesion, lung function and phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt)/rapamycin target protein (mTOR) signaling pathway in patients with idiopathic pulmonary fibrosis (IPF). Methods: 52 IPF patients (study group) admitted to our hospital from January 2021 to December 2023 were selected, and 50 healthy volunteers (control group) who underwent physical examination in our hospital during the same period were selected. The levels of serum LncRNA TUG1, miR-29a-3p, lung function parameters [total lung capacity (TLC), forced expiratory volume in the first second as a percentage of predicted value (FEV1%pred), forced vital capacity (FVC), carbon monoxide diffusion capacity as a percentage of predicted value (DLCO%pred)], high-resolution computed tomography (HRCT) score and PI3K, Akt and mTOR mRNA expression were compared in two groups. The correlation was analyzed by Pearson method. Results: The expression levels of serum LncRNA TUG1, PI3K, Akt and mTOR mRNA in study group were higher than those in control group, and the expression level of miR-29a-3p was lower than that in control group(P<0.05). TLC, FEV1%pred, FVC and DLCO%pred in study group were lower than those in control group, and HRCT score was higher than that in control group(P<0.05). The expression of serum LncRNA TUG1 was positively correlated with HRCT score, PI3K, Akt and mTOR mRNA expression, and negatively correlated with TLC, FEV1%pred, FVC and DLCO%pred (P<0.05). And miR-29a-3p was negatively correlated with HRCT score, PI3K, Akt and mTOR mRNA expression, and positively correlated with TLC, FEV1%pred, FVC and DLCO%pred (P<0.05). Conclusion: Serum LncRNA TUG1 is highly expressed, and miR-29a-3p is lowly expressed in IPF patients, the two are closely related to the degree of lung tissue lesions, the degree of lung function damage and the abnormal activation of PI3K/Akt/mTOR signaling pathway in IPF patients. |
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