| 曹新营,邢彩耐,刘丽丽,杨 光,刘馨蕊.纳-葡萄糖共转运蛋白2抑制剂调控TLRs/MyD88通路对高糖诱导HAECs自噬及凋亡的影响[J].,2024,(20):3836-3838 |
| 纳-葡萄糖共转运蛋白2抑制剂调控TLRs/MyD88通路对高糖诱导HAECs自噬及凋亡的影响 |
| The Effect of SGLT-2i Regulation of TLRs/MyD88 Pathway on High Glucose Induced Autophagy and Apoptosis in Human Aortic Endothelial Cells |
| 投稿时间：2024-04-20 修订日期：2024-05-16 |
| DOI：10.13241/j.cnki.pmb.2024.20.006 |
| 中文关键词: SGLT-2i 高糖 主动脉内皮细胞 自噬 凋亡 TLRs/MyD88通路 |
| 英文关键词: SGLT-2i High sugar Aortic endothelial cells Autophagy Apoptosis TLRs/MyD88 pathway |
| 基金项目:河北省卫健委2023年度医学科学研究课题计划项目（20231251） |
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| 中文摘要: |
| 摘要 目的：探讨纳-葡萄糖共转运蛋白2抑制剂（SGLT-2i）调控TLRs/MyD88通路对高糖诱导的人主动脉内皮细胞（HAECs）自噬及凋亡的影响。方法：选用HAECs，将细胞分为NG组（5.5 mmol/L葡萄糖）、HG组（30 mmol/L葡萄糖）和SGLT-2i+HG组（30 mmol/L葡萄糖+50 μmol/L的SGLT-2抑制剂）。检测出各细胞生长活力、细胞凋亡水平、细胞TLRs、MyD88 mRNA表达水平，细胞TLRs、MyD88、Bax、Bcl-2、Beclin 1、LC3II蛋白表达以及自噬水平。结果：与NG组相比，HG组细胞增值率显著降低（P<0.05）；与HG组相比，SGLT-2i+HG组细胞增值率显著上升（P<0.05）。12 h时24 h时时间点，与NG组相比，HG组细胞凋亡率均显著升高（P<0.05）；与HG组相比，SGLT-2i+HG组细胞凋亡率均显著下降（P<0.05）。与NG组相比，HG组凋亡相关蛋白Bax、Bcl-2蛋白表达水平升高（P<0.05）；与HG组相比，SGLT-2i+HG组细胞Bax、Bcl-2表达水平下降（P<0.05）。与NG组相比，HG组自噬相关蛋白Beclin 1、LC3II水平升高（P<0.05）；与HG组相比，SGLT-2i+HG组细胞Beclin 1、LC3II水平下降（P<0.05）。与NG组相比，HG组TLRs、MyD88蛋白表达水平升高（P<0.05）；与HG组相比，SGLT-2i+HG组细胞TLRs、MyD88表达水平下降（P<0.05）。结论：SGLT-2通过调控TLRsMyD88通路抑制高糖诱导的人主动脉内皮细胞自噬及凋亡。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of sodium glucose cotransporter 2 inhibitor (SGLT-2i) regulating the TLRs/MyD88 pathway on high glucose induced autophagy and apoptosis in human aortic endothelial cells (HAECs). Methods: Using HAECs, the cells were divided into NG group (5.5 mmol/L glucose), HG group (30 mmol/L glucose), and SGLT-2i+HG group (30 mmol/L glucose+50) μ Mol/L of SGLT-2 inhibitor. Detect the growth vitality; the level of cell apoptosis; the expression levels of TLRs and MyD88 mRNA; the levels of TLRs, MyD88, Bax, Bcl-2, Beclin 1, and LC3II in each group of cells. Results: Compared with the NG group, the cell proliferation rate of the HG group was significantly reduced (P<0.05); Compared with the HG group, the SGLT-2i+HG group showed a significant increase in cell proliferation rate (P<0.05). At 12 hours and 24 hours, compared with the NG group, the apoptosis rate of cells in the HG group was significantly increased (P<0.05); Compared with the HG group, the apoptosis rate of SGLT-2i+HG group was significantly reduced (P<0.05). Compared with the NG group, the expression levels of apoptosis related proteins Bax and Bcl-2 in the HG group increased (P<0.05); Compared with the HG group, the expression levels of Bax and Bcl-2 in cells of the SGLT-2i+HG group decreased (P<0.05). Compared with the NG group, the levels of autophagy related proteins Beclin 1 and LC3II in the HG group increased (P<0.05); Compared with the HG group, the levels of Beclin 1 and LC3II in cells of the SGLT-2i+HG group decreased (P<0.05). Compared with the NG group, the expression levels of TLRs and MyD88 proteins in the HG group increased (P<0.05); Compared with the HG group, the expression levels of TLRs and MyD88 in the SGLT-2i+HG group decreased (P<0.05). Conclusion: SGLT-2 inhibits high glucose induced autophagy and apoptosis in human aortic endothelial cells by regulating the TLRsMyD88 pathway. |
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