| 田梦影,蔡嘉洛,任木千子,邹 婷,李晓屏.金郁欢汤对抑郁状态-痰瘀互结小鼠的作用机制及BDNF/TrkB/CREB信号的动态变化分析[J].,2024,(16):3025-3031 |
| 金郁欢汤对抑郁状态-痰瘀互结小鼠的作用机制及BDNF/TrkB/CREB信号的动态变化分析 |
| The Mechanism and Dynamic Change of BDNF/TrkB/CREB Signal of Jinyuhuan Decoction on Mice with Depression and Phlegm-stasis Interformation |
| 投稿时间：2024-02-11 修订日期：2024-03-06 |
| DOI：10.13241/j.cnki.pmb.2024.16.005 |
| 中文关键词: 金郁欢汤 痰瘀互结 小鼠 海马 抑郁 BDNF/TrkB/CREB信号 |
| 英文关键词: Jinyuhuan Decoction Phlegm and blood stasis intermingled Mice The Sea Horse Depression BDNF/TrkB/CREB signal |
| 基金项目:湖南省中医药科研计划委托项目（D2023006）；湖南中医药大学中医学院院级项目（校行研字(2020)19号） |
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| 中文摘要: |
| 摘要 目的：探讨金郁欢汤对抑郁状态-痰瘀互结小鼠的作用机制及BDNF/TrkB/CREB信号的影响。方法：选择成年雄性C57BL/6J小鼠30只，根据随机数字表法分为空白组、模型组、氟西汀组、低、中、高剂量金郁欢汤组，每组5只，除空白组外其余组建立抑郁状态-痰瘀互结小鼠模型。造模完成后，氟西汀组灌胃给予3 mg/kg氟西汀悬浮灌胃，金郁欢汤低、中、高剂量组分别给予0.2 g/kg、0.5 g/kg、0.8 g/kg金郁欢汤灌胃，对照组、模型组均给予等体积的生理盐水灌胃，1次/d，连续灌胃3周。对比6组小鼠造模前、造模后、给药后的体质量、糖水偏嗜率、水平得分、垂直得分、实验不动时间、实验漂浮时间、海马组织中的BDNF/TrkB/CREB mRNA及蛋白表达水平。结果：造模后，空白组的体质量、糖水偏嗜率、水平得分、垂直得分、海马组织中的BDNF/TrkB/CREB mRNA及蛋白表达水平明显较其余5组低，实验不动、漂浮时间较其余5组高（P均<0.05）。给药后，空白组、氟西汀组、中、高剂量金郁欢汤组体质量、糖水偏嗜率明显较模型组、低剂量组高，低剂量组体质量明显较模型组高（P均<0.05）；空白组的水平、垂直得分明显较其余5组高，氟西汀组、中、高剂量金郁欢汤组以上指标明显较模型组、低剂量组高，低剂量组明显较模型组高（P均<0.05）；空白组的实验不动、漂浮时间明显较其余5组低，氟西汀组、中、高剂量金郁欢汤组实验不动、漂浮时间显较模型组、低剂量组低，低剂量组明显较模型组低（P均<0.05）；BDNF/TrkB mRNA水平、BDNF、TrkB蛋白表达水平：氟西汀组>高剂量组>中剂量组>低剂量组>空白组>模型组；其中氟西汀组、高剂量组、中剂量组三组间无差异（P>0.05），其余组间均有差异（P<0.05）；CREB mRNA水平、BDNF蛋白表达：空白组>氟西汀组>高剂量组>中剂量组>低剂量组>模型组；其中空白组、氟西汀组、高剂量组、中剂量组四组间无差异（P>0.05），其余组间均有差异（P<0.05）。结论：0.5 g/kg金郁欢汤可明显改善抑郁状态-痰瘀互结小鼠的抑郁行为，增强海马神经元的修复、再生，可能与其可上调小鼠海马组织的BDNF/TrkB/CREB信号通路相关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of Jinyuhuan Decoction on depression and phlegm-stasis in mice and the effects of BDNF/TrkB/CREB signal. Methods: Thirty adult male C57BL / 6J mice were selected and divided into blank group, model group, model group, fluoxetine group, low, medium and high dose Jin Yu Tang group. In each group, the mouse model of depression-sputum stasis was established. After the mold production, fluoxetine group was given 3 mg/kg fluoxetine in suspension, low-, medium-and high-dose group was given 0.2 g/kg, 0.5 g/kg, 0.8 g/kg Jinyu soup, control group and model group were given equal volume of normal saline, 1 time/d for 3 weeks. Body mass, sugar water bias, horizontal score, vertical score, experimental motionless time, experimental time, floating time, BDNF / TrkB / CREB mRNA and protein expression levels in the hippocampus. Results: After modeling, the body mass, sugar and water preference, horizontal score, vertical score, BDNF/TrkB/CREB mRNA and protein expression levels in hippocampus of blank group were significantly lower than those of the other 5 groups, and the experimental immovement and floating time were higher than those of the other 5 groups (all P<0.05). After administration, body weight and sugar and water preference in blank group, fluoxetine group, medium and high dose Jinyuhuan tang group were higher than those in model group and low dose group, and body weight in low dose group was higher than that in model group (all P<0.05). The horizontal and vertical scores of blank group were higher than those of the other 5 groups, the above indexes of fluoxetine group, medium and high dose Jinyuhuan tang group were higher than those of model group and low dose group, and the low dose group was higher than that of model group (P <0.05). The experimental immobility and floating time of the blank group was significantly lower than that of the other 5 groups, the experimental immobility and floating time of fluoxetine group, medium and high dose Jinyuhuan tang group was lower than that of model group and low dose group, and the low dose group was lower than that of model group (all P<0.05). BDNF/TrkB mRNA level, BDNF, TrkB protein expression level: fluoxetine group > high dose group > medium dose group > low dose group > blank group > model group; There was no difference among fluoxetine group, high dose group and medium dose group (P>0.05), but there was difference among other groups (P<0.05). CREB mRNA level and BDNF protein expression: blank group > fluoxetine group > high dose group > medium dose group > low dose group > model group; There were no differences among blank group, fluoxetine group, high dose group and medium dose group (P>0.05), but there were differences among other groups (P<0.05). Conclusion: 0.5 g/kg Jinyu Huan Decoction can significantly improve the depressive behavior and enhance the repair and regeneration of hippocampus neurons, which may be related to the up-regulation of BDNF/TrkB/CREB signaling pathway in hippocampus of mice. |
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