| 贾世炎,刘伟仙,李裕婷,彭彩霞,付远亮.LncRNA MALAT1、miR-145表达与早产儿视网膜病变严重程度的关系研究[J].,2024,(12):2377-2382 |
| LncRNA MALAT1、miR-145表达与早产儿视网膜病变严重程度的关系研究 |
| Study on the Relationship between the Expression of LncRNA MALAT1 and miR-145 and the Severity of Retinopathy of Prematurity |
| 投稿时间：2024-01-10 修订日期：2024-01-31 |
| DOI：10.13241/j.cnki.pmb.2024.12.035 |
| 中文关键词: 早产儿 视网膜病变 LncRNA MALAT1 miR-145 病情程度 |
| 英文关键词: Premature infant Retinopathy LncRNA MALAT1 MiR-145 Severity |
| 基金项目:海南省科技厅重点研发项目(ZDYF2019184) |
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| 中文摘要: |
| 摘要 目的：探讨长链非编码核糖核酸（RNA）肺腺癌转移相关转录本1（LncRNA MALAT1）、微小RNA-145（miR-145）表达与早产儿视网膜病变（ROP）严重程度的关系。方法：选择2021年1月至2023年12月我院收治的ROP早产儿82例，根据病情严重程度分为重度组、轻度组，例数分别为为38例、44例。另选择同期我院健康早产儿40例纳入对照组。比较三组血清LncRNA MALAT1、miR-145表达，应用Pearson相关性分析血清LncRNA MALAT1、miR-145表达与ROP严重程度的关系。早产儿ROP的影响因素应用多因素Logistic回归模型分析。血清LncRNA MALAT1、miR-145对早产儿ROP的预测价值应用受试者工作特征（ROC）曲线分析。结果：重度组血清LncRNA MALAT1表达显著高于轻度组和对照组，miR-145表达显著低于轻度组和对照组；轻度组LncRNA MALAT1表达显著高于对照组，miR-145表达显著低于对照组（P<0.05）。血清miR-145表达与ROP严重程度呈负相关（r=-0.412，P<0.05），LncRNA MALAT1表达与ROP严重程度呈正相关（r=0.434，P<0.05）。三组胎龄、出生体重<1500 g构成比、出生1min Apgar评分<7分、出生5 min Apgar评分<7分、母亲妊娠期糖尿病发生率有统计学意义（P<0.05）。LncRNA MALAT1升高、miR-145降低、胎龄≤31周、出生体重<1500 g、母亲妊娠期糖尿病是早产儿ROP发生的危险因素（P<0.05）。血清LncRNA MALAT1、miR-145对ROP严重程度的预测价值具有较高的敏感度、特异度，联合检测对ROP严重程度的曲线下面积（AUC）大于上述指标单独检测。结论：ROP早产儿血清LncRNA MALAT1表达升高、miR-145表达降低与ROP严重程度加重有关，血清LncRNA MALAT1联合miR-145检测对ROP严重程度预测具有一定价值。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between the expression of long non-coding ribonucleic acids (RNA) lung adenocarcinoma metastasis-associated transcript 1 (LncRNA MALAT1) and microRNA-145 (miR-145) and the severity of retinopathy of prematurity (ROP). Methods: 82 premature infants with ROP admitted to our hospital from January 2021 to December 2023 were selected, according to the severity of the condition, there are 38 cases in the severe group and 44 cases in the mild group. Another 40 healthy premature infants in our hospital during the same period were selected as control group. The expressions of serum LncRNA MALAT1 and miR-145 were compared in three groups, the relationship between the expressions of serum LncRNA MALAT1 and miR-145 and the severity of ROP was analyzed by Pearson correlation. The influencing factors of ROP in premature infants were analyzed by univariate and multivariate Logistic regression models. The predictive value of serum LncRNA MALAT1 and miR-145 for ROP in premature infants was analyzed by receiver operating characteristic (ROC) curve. Results: The expression of serum LncRNA MALAT1 in severe group was significantly higher than that in mild group and control group, and the expression of miR-145 was significantly lower than that in mild group and control group. The expression of LncRNA MALAT1 in mild group was significantly higher than that in control group, and the expression of miR-145 was significantly lower than that in control group (P<0.05). Serum miR-145 expression was negatively correlated with the severity of ROP (r=-0.412, P<0.05), and LncRNA MALAT1 expression was positively correlated with the severity of ROP (r=0.434, P<0.05). Three groups of gestational age, birth weight<1500 g constituent ratio, birth 1min Apgar score<7 points, birth 5min Apgar score<7 points, the incidence of maternal gestational diabetes was statistically significant(P<0.05). Elevated LncRNA MALAT1, decreased miR-145, gestational age≤31 weeks, birth weight<1500 g, and maternal gestational diabetes were risk factors for ROP in preterm infants (P<0.05). Serum LncRNA MALAT1 and miR-145 had high sensitivity and specificity in predicting the severity of ROP, the area under the curve (AUC) of combined detection of ROP severity was greater than that of the above indicators alone. Conclusion: The increase of serum LncRNA MALAT1 expression and the decrease of miR-145 expression in ROP premature infants are relate to the aggravation of ROP severity, the detection of serum LncRNA MALAT1 combine with miR-145 has certain value in predicting the severity of ROP. |
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