文章摘要
血清lncRNA MEG3、miR-424-5p水平与急性呼吸窘迫综合征患儿病情严重程度和预后的关系
Relationship Between Serum lncRNA MEG3 and miR-424-5p Levels and the Severity and Prognosis of Children With Acute Respiratory Distress Syndrome
投稿时间:2024-09-09  修订日期:2024-09-09
DOI:
中文关键词: 急性呼吸窘迫综合征  lncRNA MEG3  miR-424-5p  病情严重程度  预后
英文关键词: Acute respiratory distress syndrome  lncRNA MEG3  miR-424-5p  Severity of disease  Prognosis
基金项目:江苏省妇幼健康科研项目(F202065);南通市科技项目(MS22022026)
作者单位邮编
洪菲* 南通大学第二附属医院 226000
摘要点击次数: 86
全文下载次数: 0
中文摘要:
      目的:分析血清长链非编码RNA?MEG3(lncRNA?MEG3)、微小核糖核酸-424-5p(miR-424-5p)在急性呼吸窘迫综合征(ARDS)患儿中的表达及与其病情严重程度和预后的关系。方法:选取2021年6月至2023年6月本院收治的263例ARDS患儿作为观察组,观察组依据氧合指数(OI)分为轻度组(n=101)、中度组(n=105)和重度组(n=57),依据预后情况分为存活组(n=167)和死亡组(n=96);另选取同期于本院健康体检儿童160例作为对照组。对比对照组和观察组血清lncRNA?MEG3、miR-424-5p水平;比较轻度组、中度组和重度组血清lncRNA?MEG3、miR-424-5p水平;Pearson相关性分析血清lncRNA?MEG3、miR-424-5p水平与患儿病情严重程度的相关性;单因素和多因素COX风险回归模型分析影响ARDS患儿死亡的影响因素;受试者工作特征(ROC)曲线分析血清lncRNA?MEG3、miR-424-5p对ARDS患儿死亡的预测价值。结果:与对照组比较,观察组血清lncRNA?MEG3水平明显更低,miR-424-5p水平明显更高(P<0.05);与轻度组和中度组比较,重度组血清lncRNA?MEG3水平明显更低,miR-424-5p水平、OI明显更高(P<0.05);与轻度组比较,中度组lncRNA?MEG3水平显著降低,miR-424-5p水平、OI显著升高(P<0.05);血清lncRNA?MEG3与OI呈负相关,血清miR-424-5p与OI呈正相关(P<0.05);OI、miR-424-5p、白介素-6(IL-6)、白介素-17(IL-17)、肿瘤坏死因子-α(TNF-α)是影响ARDS患儿预后的独立危险因素,lncRNA?MEG3是影响ARDS患儿预后的独立保护因素(P<0.05);血清lncRNA?MEG3、miR-424-5p对ARDS患儿死亡均有一定预测价值,但联合检测预测的曲线下面积(AUC)为0.869高于二者单独预测的0.714、0.727(P<0.05)。结论:血清lncRNA?MEG3、miR-424-5p与ARDS患儿病情严重程度具有相关性,并且可以作为ARDS患儿预后的评估指标。
英文摘要:
      Objective: To analyze the expression of serum long non-coding RNA MEG3 (lncRNA MEG3) and microRNA-424-5p (miR-424-5p) in children with acute respiratory distress syndrome (ARDS) and their relationship with the severity and prognosis of the disease. Methods: 263 children with ARDS admitted to our hospital from June 2021 to June 2023 were selected as observation group, observation group were divided into mild group (n=101), moderate group (n=105) and severe group (n=57) according to the oxygenation index (OI), observation group were divided into survival group (n=167) and death group (n=96) according to the prognosis. Another 160 children who had health checkups in our hospital during the same period were selected as control group. The levels of serum lncRNA MEG3 and miR-424-5p in control group and observation group were compared. The levels of serum lncRNA MEG3 and miR-424-5p in mild group, moderate group and severe group were compared. The correlation between serum lncRNA MEG3, miR-424-5p levels and the severity of the disease in children were analyzed by Pearson correlation analysis. The influencing factors of death in children with ARDS were analyzed by univariate and multivariate COX risk regression models. The predictive value of serum lncRNA MEG3, miR-424-5p for death in children with ARDS were analyzed by receiver operating characteristic (ROC) curve. Results: Compared with control group, the serum lncRNA MEG3 level in observation group was significantly lower, and miR-424-5p level was significantly higher (P<0.05). Compared with mild group and moderate group, the serum lncRNA MEG3 level in severe group was significantly lower, the miR-424-5p level and OI were significantly higher (P<0.05). Compared with mild group, the level of lncRNA MEG3 in moderate group was significantly decreased, and the level of miR-424-5p and OI were significantly increased (P<0.05). Serum lncRNA MEG3 was negatively correlated with OI, and serum miR-424-5p was positively correlated with OI (P<0.05). OI, miR-424-5p, interleukin-6 (IL-6), interleukin-17 (IL-17) and tumor necrosis factor-α (TNF-α) were independent risk factors affecting the prognosis of children with ARDS, and lncRNA MEG3 was an independent protective factor affecting the prognosis of children with ARDS (P<0.05). Serum lncRNA MEG3 and miR-424-5p had certain predictive value for death in children with ARDS, and the area under the curve (AUC) predicted by combined detection was 0.869, which was higher than 0.714 and 0.727 predicted by the two alone (P<0.05). Conclusion: Serum lncRNA MEG3 and miR-424-5p are correlated with the severity of ARDS in children, and can be used as prognostic indicators for children with ARDS.
View Fulltext   查看/发表评论  下载PDF阅读器
关闭