| 张佳佳,许良飞,金佩佩,肖雨寒,章 菊.结直肠癌小鼠肠道菌群变化与巨噬细胞极化的相关性分析[J].,2024,(9):1633-1638 |
| 结直肠癌小鼠肠道菌群变化与巨噬细胞极化的相关性分析 |
| Correlation Analysis between Changes in Intestinal Flora and Macrophage Polarization in Mice with Colorectal Cancer |
| 投稿时间：2023-10-16 修订日期：2023-11-12 |
| DOI：10.13241/j.cnki.pmb.2024.09.006 |
| 中文关键词: 结直肠癌 肠道菌群 巨噬细胞极化 宏基因测序 差异分析 |
| 英文关键词: Colorectal cancer Intestinal flora Macrophage polarization Macrogene sequencing Difference analysis |
| 基金项目:安徽省自然科学基金项目（2208085MH246） |
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| 中文摘要: |
| 摘要 目的：宏基因测序分析结直肠癌小鼠肠道菌群变化与巨噬细胞极化的相关性。方法：将3周龄APCmin/+小鼠和野生型C57/6J小鼠分成结直肠癌组（CRC组，n=8）和正常对照组（NC组，n=7），高脂饮食喂养8周收集粪便宏基因测序分析肠道菌群变化，免疫组化（IHC）检测肿瘤组织M2巨噬细胞极化。结果：HE病理显示CRC组小鼠造模成功，肿瘤数量显著多于NC组（P<0.05）。α多样性显示CRC组菌群多样性高于NC组，菌群丰富度无显著差异；β多样性显示两组间菌群明显分离。Venn图显示两组间共有物种1620种，NC组和CRC组特有物种分别为109、191种，门水平上两组间以厚壁菌门（74.04％vs 87.56％）和拟杆菌门（20.33％ vs 6.42％）为主；种水平Metastats分析显示艰难梭菌（Clostridioides difficile_A）、弗雷特肯氏菌（Duncaniella freteri）、粪副拟杆菌（Parabacteroides merdae）、内脏拟杆菌（Odoribacter splanchnicus）在两组间具有显著差异（P<0.05）；KEGG功能分析显示CRC组核糖体、糖酵解及糖原生成、氧化磷酸化等通路显著富集；NC组神经活性配体受体相互作用、人类T细胞白血病病毒感染、胆固醇代谢通路显著富集；IHC分析显示CRC组M2巨噬细胞CD206含量高于NC组，Pearson相关性分析显示CRC组内脏拟杆菌丰度与CD206呈正相关（r=0.7799）。结论：CRC小鼠肠道菌群发生显著变化并存在功能差异，不同差异菌群与巨噬细胞极化可能存在一定的相关性。 |
| 英文摘要: |
| ABSTRACT Objective: Correlation between changes in intestinal flora and macrophage polarization in colorectal cancer mice analyzed by macrogene sequencing. Methods: 3-week-old APCmin/+ mice and wild-type C57/6J mice were divided into colorectal cancer group (CRC group, n=8) and normal control group (NC group, n=7), which were given high-fat diet fed for 8 weeks, collect feces and analysis of intestinal flora changes by macrogenetic sequencing, Immunohistochemical (IHC) detect M2 macrophage polarization in tumor tissue. Results: HE pathology shows successful modeling in CRC group mice, significantly more tumors than NC group (P<0.05). The α-diversity showed that CRC group flora diversity higher than NC group, flora richness no significant difference; diversity showed clear separation of the flora between two groups. The Venn diagram demonstrated a total of 1,620 species between the two groups, with 109 species endemic to the NC group and 191 species endemic to the CRC group respectively. At the phylum level, the CRC and NC groups were dominated by the Firmicutes (74.04% vs. 87.56%) and the Bacteroidota (20.33% vs. 6.42%); at the species level, Metastats analysis showed that the species of Clostridioides difficile_A, Duncaniella freteri, Parabacteroides merdae, and Odoribacter splanchnicus were significantly different between the two groups (P<0.05); KEGG functional analysis shows that in the CRC group Ribosome, Glycolysis, Gluconeogenesis, Oxidative phosphorylation pathway were significantly enriched in the CRC group; Neuroactiveligand receptorinteraction, Human T_cellleukemiavirus1infection, Cholesterol metabolism pathway were significantly enriched in the NC group; immunohistochemical analyses showed that M2 macrophage CD206 content was higher in the CRC, Pearson correlation analyses showed that abundance of Odoribacter splanchnicus was positively correlated to the CD206 (r=0.7799). Conclusion: Significant changes and functional differences in the intestinal flora of colorectal cancer mice, there may be a correlation between the differential flora and macrophage polarization. |
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