| 李 雨,李小风,张 杨,王 鹏,唐仁哲.木霉菌醇通过p53信号通路对胃癌大鼠的肠道微生态、胃肠道功能及免疫功能的影响[J].,2024,(8):1428-1432 |
| 木霉菌醇通过p53信号通路对胃癌大鼠的肠道微生态、胃肠道功能及免疫功能的影响 |
| Effects of Trichoderma on Intestinal Microecology, Gastrointestinal Function and Immune Function of Gastric Cancer Rats through p53 Signaling Pathway |
| 投稿时间：2023-10-23 修订日期：2023-11-19 |
| DOI：10.13241/j.cnki.pmb.2024.08.004 |
| 中文关键词: 胃癌大鼠 木霉菌醇 p53信号通路 肠道微生态 胃肠道功能 免疫功能 |
| 英文关键词: Gastric cancer rats Trichoderma alcohol p53 signaling pathway Intestinal microecology Gastrointestinal function Immune function |
| 基金项目:黑龙江省自然科学基金项目（NO.LH2023H059） |
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| 中文摘要: |
| 摘要 目的：分析木霉菌醇通过p53信号通路对胃癌大鼠的肠道微生态、胃肠道功能及免疫功能的影响。方法：随机将40只雄性SD大鼠分为模型组、木霉菌醇低剂量组、木霉菌醇中剂量组和木霉菌醇高剂量组，每组各10只；另选取10只健康雄性大鼠分为空白组。空白组、模型组制模成功后给予等量生理盐水灌胃，木霉菌醇低、中、高剂量组分别给予20、40、60 mg/kg灌胃。采用Western blot检测p53蛋白表达水平；采用16S rDNA测定法检测肠道菌群变化情况；采用酶联免疫吸附法（ELISA）检测血清胃肠道功能指标水平；采用流式细胞仪检测外周血T淋巴细胞亚群。结果：模型组、木霉菌醇（低、中、高剂量）组p53蛋白表达水平比空白组低，木霉菌醇中剂量组p53蛋白水平比模型组、木霉菌醇（低、高剂量）组高（P＜0.05）。模型组、木霉菌醇（低、中、高剂量）组厚壁菌门、拟杆菌门、变形菌门水平明显高于空白组高，木霉菌醇中剂量组厚壁菌门、拟杆菌门、变形菌门水平明显低于模型组、木霉菌醇（低、高剂量）组（P＜0.05）。模型组、木霉菌醇（低、中、高剂量）组胃泌素、胃动素水平明显比空白组低，木霉菌醇中低剂量组胃泌素（GAS）、胃动素（MTL）水平明显比模型组、木霉菌醇（低、高剂量）组（P＜0.05）。与空白组比较，模型组、木霉菌醇（低、中、高剂量）组CD3+、CD4+水平较高，CD8+水平较低；与模型组、木霉菌醇（低、高剂量）组比较，木霉菌醇中低剂量组CD3+、CD4+水平较低，CD8+水平较高（P＜0.05）。结论：木霉菌醇可有效调节胃癌大鼠肠道菌群，并增强其免疫功能，促使肠道功能恢复，且中剂量应用效果更佳。 |
| 英文摘要: |
| ABSTRACT Objective: Effects of Trichoderma on intestinal microecology, gastrointestinal function and immune function of gastric cancer rats through p53 signaling pathway. Methods: 40 male SD rats were randomly divided into model group, low dose group, middle dose group and high dose group, 10 rats in each group. Another 10 healthy male rats were selected and divided into blank group. After successful modeling, the blank group and the model group were given the same amount of normal saline by gavage, and the low, medium and high dose groups of trichoderma alcohol were given 20, 40 and 60 mg / kg by gavage. The expression of p53 protein was detected by Western blot. The changes of intestinal flora were detected by 16 S rDNA assay. The levels of serum gastrointestinal function indexes were detected by enzyme-linked immunosorbent assay (ELISA). Peripheral blood T lymphocyte subsets were detected by flow cytometry. Results: The expression level of p53 protein in the model group and the low, medium and high dose groups was lower than that in the blank group, and the p53 protein level in the medium dose group was higher than that in the model group and the low and high dose groups (P<0.05). The levels of Firmicutes, Bacteroidetes and Proteobacteria in the model group and Trichoderma alcohol (low, medium and high dose) groups were significantly higher than those in the blank group. The levels of Firmicutes, Bacteroidetes and Proteobacteria in the medium dose group were significantly lower than those in the model group and Trichoderma alcohol (low and high dose) groups (P<0.05). The levels of gastrin and motilin in the model group and the trichoderma alcohol (low, medium and high dose) group were significantly lower than those in the blank group, and the levels of gastrin (GAS) and motilin (MTL) in the middle and low dose group of trichoderma alcohol were significantly higher than those in the model group and the trichoderma alcohol (low and high dose) group (P<0.05). Compared with the blank group, the levels of CD3+ and CD4+ in the model group and the Trichoderma alcohol (low, medium and high dose) groups were higher, and the level of CD8+ was lower. Compared with the model group and the low-dose and high-dose groups, the levels of CD3+ and CD4+ in the low-dose group were lower, and the level of CD8+ was higher (P<0.05). Conclusion: Trichoderma alcohol can effectively regulate the intestinal flora of gastric cancer rats, enhance their immune function, and promote the recovery of intestinal function, and the medium dose application effect is better. |
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