| Objective To analyze the expression and clinical significance of serum exosomes miR-122 and miR-874 in patients with colorectal cancer. Methods 155 CRC patients (CRC group) admitted to the The Affiliated Hospital of Southwest Medical University from February 2019 to January 2021 and 120 healthy examined patients in the same period (healthy group) were selected, the expression of serum exosomal miR-122 and miR-874 were determined by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR), furthermore the correlation between the clinicopathological characteristics of colorectal cancer and the expression of serum exosomal miR-122 and miR-874 from CRC patient was examined. The mean expression levels of serum exosomal miR-122 and miR-874 from CRC patient were used as the critical value, colorectal cancer patients were divided into low expression group and high expression group, 3 years after follow-up, the relationship between the expression of serum exosomal miR-122 and miR-874 and overall survival (OS) and disease-free survival (DFS) were analyzed by Kaplan-Meier curve, the value of serum exosomal miR-122, miR-874 and their combination in predicting the diagnosis of colorectal cancer were analyzed by receiver operating characteristic (ROC) curve. Results Serum exosomal miR-122 was expressed at a greater level in the CRC group compared to the healthy group, whereas miR-874 was expressed at a lower level (P<0.05). CRC patients with an invasion depth of T3+T4, TNM stage III, lesion diameter ≥3cm, moderately differentiated, and lymph node metastasis had higher expression of serum exosome miR-122 and lower expression of miR-874 than patients with an invasion depth of T1+T2, TNM stage Ⅰ-Ⅱ, lesion diameter < 3cm, highly differentiated, and lymph node metastasis, respectively (P<0.05). Point two column correlation showed that, the infiltration depth, TNM stage, lesion diameter, differentiation degree and lymph node metastasis status of colorectal cancer patients were positively correlated with the expression of serum exosomal miR-122 (P<0.05), and negatively correlated with the expression of miR-874 (P<0.05). The 3-year overall survival rate and disease-free survival rate of 155 patients with colorectal cancer were 75.48% (117/155) and 62.58% (97/155), respectively. The OS and DFS of miR-122 high expression group were 30.592 months and 28.632 months, respectively, which were lower than 32.962 months and 32.266 months of miR-122 low expression group (P<0.05). The OS and DFS of miR-874 low expression group were 29.403 months and 27.778 months, respectively, which were lower than 33.470 months and 32.627 months of miR-874 high expression group (P<0.05). ROC curve showed that, the area under the curve (AUC) of serum exosomal miR-122, miR-874 and their combined detection in diagnoseing colorectal cancer were 0.869, 0.825 and 0.921, respectively. Conclusion The expression of serum exosomal miR-122 increased and miR-874 decreased in patients with colorectal cancer, The expression of which is related to the clinicopathological features and survival time of patients, and have a high diagnostic value. |
